Keshgegian A A, Kline T S
Am J Clin Pathol. 1984 Nov;82(5):586-9. doi: 10.1093/ajcp/82.5.586.
The authors investigated the usefulness of immunoperoxidase demonstration of prostate-specific acid phosphatase (PAP) in determining the tissue of origin for carcinoma in aspiration biopsy cytology (ABC) specimens. Antisera from a commercial kit were applied using standard technics directly to Papanicoloau-stained slides. PAP was diffusely positive in numerous tumor cells of 27/28 moderately or poorly differentiated and 17/18 well-differentiated primary prostatic carcinomas. A negative result upon substituting the primary antiserum confirmed the reaction specificity in 18 cases with an additional available slide. PAP was demonstrable in corresponding histologic sections of 16/17 moderately or poorly differentiated and 18/18 well-differentiated carcinomas. Only 2 of 22 nonprostatic carcinomas (both of breast origin) showed very rare positive cells. Four ABC specimens of metastatic prostatic carcinoma were diffusely positive. Thus, diffuse staining of PAP by immunoperoxidase in tumor cells of ABC specimens gives high sensitivity and specificity for prostate as the tissue of origin.
作者研究了免疫过氧化物酶法显示前列腺特异性酸性磷酸酶（PAP）在确定针吸活检细胞学（ABC）标本中癌组织来源方面的实用性。使用标准技术将市售试剂盒中的抗血清直接应用于巴氏染色玻片。在27/28例中分化或低分化以及17/18例高分化原发性前列腺癌的众多肿瘤细胞中，PAP呈弥漫性阳性。在18例有额外可用玻片的病例中，用替代的一抗得到阴性结果证实了反应的特异性。在16/17例中分化或低分化以及18/18例高分化癌的相应组织学切片中可显示PAP。22例非前列腺癌（均为乳腺来源）中只有2例显示极罕见的阳性细胞。4例转移性前列腺癌的ABC标本呈弥漫性阳性。因此，ABC标本肿瘤细胞中PAP的免疫过氧化物酶弥漫性染色对前列腺作为组织来源具有高敏感性和特异性。
