Katz R L, Raval P, Brooks T E, Ordóñez N G
Diagn Cytopathol. 1985 Jan-Mar;1(1):28-32. doi: 10.1002/dc.2840010108.
The efficacy of immunocytochemical staining for prostate-specific antigen (PSA) and prostate-specific acid phosphatase (PSAP) was studied in aspiration biopsy specimens from 19 patients. Eighteen patients had prostatic carcinoma and one had hyperplasia of prostate. Specimens were obtained from both the primary tumors and metastatic sites. Immunoperoxidase staining was performed on alcohol-fixed cytology smears (some prepared up to 9 years previously) using appropriate antisera followed by an avidin-biotinylated horseradish peroxidase complex. Results were scored according to the percentage and intensity of positively stained malignant cells. Corresponding histologic specimens were stained and scored in a similar fashion. Correlations were made between the staining characteristics of the tumor markers and grade of tumor, using the University of Texas M.D. Anderson Hospital classification of prostate carcinoma. Overall there was good correlation between cytologic and histologic specimens for the presence of PSA and PSAP, although metastases tended to show fewer positively stained cells than the primary tumor. There was no relationship between tumor grade and percentage of positively stained cells. Ninety-three percent of aspirated primary and secondary prostatic tumors stained positively for PSAP compared with 81% for PSA. In one of 3 patients, negative staining of neoplastic cells by both PSAP and PSA was helpful in confirming the existence of a second primary tumor.
对19例患者穿刺活检标本中前列腺特异性抗原（PSA）和前列腺特异性酸性磷酸酶（PSAP）免疫细胞化学染色的效果进行了研究。18例患者患有前列腺癌，1例患有前列腺增生。标本取自原发性肿瘤和转移部位。使用合适的抗血清，随后用抗生物素蛋白-生物素化辣根过氧化物酶复合物，对酒精固定的细胞学涂片（有些涂片制备时间长达9年）进行免疫过氧化物酶染色。根据阳性染色恶性细胞的百分比和强度对结果进行评分。相应的组织学标本以类似方式染色和评分。利用德克萨斯大学MD安德森医院的前列腺癌分类法，对肿瘤标志物的染色特征与肿瘤分级之间进行相关性分析。总体而言，PSA和PSAP存在时，细胞学和组织学标本之间具有良好的相关性，尽管转移灶中阳性染色细胞往往比原发性肿瘤少。肿瘤分级与阳性染色细胞百分比之间无相关性。93%的原发性和继发性前列腺穿刺肿瘤PSAP染色呈阳性，而PSA染色呈阳性的比例为81%。在3例患者中的1例中，PSAP和PSA对肿瘤细胞均呈阴性染色有助于确认第二原发性肿瘤的存在。
