副球孢子菌病中的酶联免疫吸附测定（ELISA）。与对流免疫电泳和红细胞免疫测定的比较。
Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis. Comparison with counterimmunoelectrophoresis and erythro-immunoassay.
作者信息
de Camargo Z P, Guesdon J L, Drouhet E, Improvisi L
出版信息
Mycopathologia. 1984 Oct 30;88(1):31-7. doi: 10.1007/BF00439292.
An enzyme-linked immunosorbent assay (ELISA) for detection and quantification of antibodies anti-Paracoccidioides brasiliensis is described. Polystyrene plates have been used as solid phase to absorb P. brasiliensis metabolic yeast phase antigen. Twenty sera of proven paracoccidioidomycosis, 11 of histoplasmosis due Histoplasma capsulatum, 20 of aspergillosis and 20 human normal sera were tested. Ninety-five percent of the paracoccidioidomycosis sera had O.D. superior to 0.150 (from 0.163 to 2.650) at 1/400 serum dilution. ELISA assay was compared with counterimmunoelectrophoresis and erythro-immunoassay tests; a correlation was observed only with erythro-immunoassay. ELISA test should give new perspectives for the serodiagnosis of paracoccidioidomycosis.
本文描述了一种用于检测和定量抗巴西副球孢子菌抗体的酶联免疫吸附测定（ELISA）方法。已使用聚苯乙烯板作为固相来吸附巴西副球孢子菌代谢酵母相抗原。检测了20份经证实的副球孢子菌病血清、11份由荚膜组织胞浆菌引起的组织胞浆菌病血清、20份曲霉病血清和20份正常人血清。在血清稀释度为1/400时，95%的副球孢子菌病血清的光密度值高于0.150（范围为0.163至2.650）。将ELISA测定与对流免疫电泳和红细胞免疫测定试验进行了比较；仅观察到与红细胞免疫测定试验有相关性。ELISA试验应为副球孢子菌病的血清学诊断提供新的视角。
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