Scott E N, Muchmore H G, Felton F G
Sabouraudia. 1981 Dec;19(4):257-65. doi: 10.1080/00362178185380431.
In this investigation, enzyme-linked immunosorbent assay (ElISA) procedures were used to study the time of appearance and the duration of demonstrable antigen and antibody in body fluids of mice with disseminated cryptococcosis. The ELISA antigen procedure detected cryptococcal capsular polysaccharide (CCP) in the serum and urine of infected mice 3 days after infection--4 days before it could be demonstrated by the latex agglutination procedure. ELISA-reactive antibody was present throughout the course of infection (mean death time, 32 days), whereas antibody was not detected by whole cell agglutination after day 20. High serum concentrations of CCP (titers to 64,000) persisted throughout the course of infection, while antibody declined to low levels with progression of disease. ELISA provides a sensitive system for quantitation and monitoring of antigen (CCP) processing and clearance (or storage), and for cryptococcal antibody formation in progressive cryptococcosis.
在本研究中，采用酶联免疫吸附测定（ELISA）方法，研究播散性隐球菌病小鼠体液中可检测到的抗原和抗体出现的时间及持续时间。ELISA抗原检测方法在感染后3天即在感染小鼠的血清和尿液中检测到隐球菌荚膜多糖（CCP），比乳胶凝集法能检测到的时间早4天。ELISA反应性抗体在整个感染过程中均存在（平均死亡时间为32天），而在第20天后全细胞凝集法未检测到抗体。高血清浓度的CCP（滴度达64,000）在整个感染过程中持续存在，而抗体随疾病进展降至低水平。ELISA为定量和监测进行性隐球菌病中抗原（CCP）的处理和清除（或储存）以及隐球菌抗体形成提供了一个灵敏的系统。
