Ryukyu Animal Medical Center, Yone 5-27, Tomigusuku, Okinawa, 901-0224, Japan.
The United Graduate School of Agriculture Sciences, Kagoshima University, Kagoshima, 890-0065, Japan.
Mycopathologia. 2018 Oct;183(5):793-803. doi: 10.1007/s11046-018-0295-0. Epub 2018 Aug 30.
Paracoccidioidomycosis ceti is a cutaneous disease of cetaceans caused by uncultivated Paracoccidioides brasiliensis or Paracoccidioides spp. Serological cross-reactions between paracoccidioidomycosis ceti and paracoccidioidomycosis, paracoccidioidomycosis and histoplasmosis, and paracoccidioidomycosis and coccidioidomycosis have been reported before. The present study aimed to detect immunohistochemical cross-reaction between antibodies to Paracoccidioides sp. and Histoplasma capsulatum, and vice versa. Thirty murine sera, obtained from experimental infections of 6 isolates of H. capsulatum, were reacted with paraffin-embedded yeast-form cells of Paracoccidioides sp. derived from a case of paracoccidioidomycosis ceti in Japan. The murine sera were also reacted with human isolates of H. capsulatum yeast cells, with P. brasiliensis yeast cells, and with fungal cells of Coccidioides posadasii. Three dolphins' sera from cases of paracoccidioidomycosis ceti, two human sera from patients with paracoccidioidomycosis, and a serum from a healthy person with a history of coccidioidomycosis were used in order to determine that the tested fungal cells reacted properly. Sera derived from mice infected with an isolate of H. capsulatum reacted positively against yeast cells of Paracoccidioides sp., yeast cells of P. brasiliensis, and fungal cells of C. posadasii, while those derived from other strains were negative. The present study recorded for the first time the cross-reaction between the yeast cells of H. capsulatum and antibodies against Paracoccidioides spp., the yeast cells of Paracoccidioides sp. and antibodies against H. capsulatum, the yeast cells of Paracoccidioides sp. and antibodies against Coccidioides sp., and fungal cells of C. posadasii and antibodies against Paracoccidioides spp.
海生型巴西副球孢子菌病是一种发生于鲸目动物的皮肤疾病，由未培养的巴西副球孢子菌或副球孢子菌属种引起。此前已有报道称海生型巴西副球孢子菌病与内脏利什曼病、球孢子菌病和粗球孢子菌病之间存在血清学交叉反应。本研究旨在检测针对巴西副球孢子菌和荚膜组织胞浆菌的抗体之间的免疫组织化学交叉反应，反之亦然。从 6 株荚膜组织胞浆菌分离株的实验感染中获得的 30 只鼠血清与来自日本海生型巴西副球孢子菌病病例的巴西副球孢子菌石蜡包埋酵母细胞反应。鼠血清还与荚膜组织胞浆菌的人类分离株、巴西副球孢子菌的酵母细胞和粗球孢子菌的真菌细胞反应。来自 3 例海生型巴西副球孢子菌病的 3 只海豚血清、来自 2 例巴西副球孢子菌病患者的 2 例人血清和 1 例曾患粗球孢子菌病的健康人血清用于确定所测试的真菌细胞反应正常。感染荚膜组织胞浆菌分离株的鼠血清对巴西副球孢子菌的酵母细胞、巴西副球孢子菌的酵母细胞和粗球孢子菌的真菌细胞呈阳性反应，而来自其他菌株的血清则呈阴性反应。本研究首次记录了荚膜组织胞浆菌的酵母细胞与针对巴西副球孢子菌属种的抗体之间的交叉反应、巴西副球孢子菌的酵母细胞与针对荚膜组织胞浆菌的抗体之间的交叉反应、巴西副球孢子菌的酵母细胞与针对粗球孢子菌属种的抗体之间的交叉反应，以及粗球孢子菌的真菌细胞与针对巴西副球孢子菌属种的抗体之间的交叉反应。
