Camargo Z P, Gesztesi J L, Saraiva E C, Taborda C P, Vicentini A P, Lopes J D
Disciplina de Biologia Celular, Escola Paulista de Medicina, Brasil.
J Clin Microbiol. 1994 Oct;32(10):2377-81. doi: 10.1128/jcm.32.10.2377-2381.1994.
Four murine monoclonal antibodies (MAbs 17C, 21A, 21F, and 32B) raised against the 43-kDa glycoprotein of Paracoccidioides brasiliensis were tested in a capture enzyme immunoassay (EIA) for the detection of specific human anti-gp43 immunoglobulin G in patients with paracoccidioidomycosis (PCM). All MAbs reacted similarly in the assay. These MAbs, which detected anti-gp43 at levels of as low as 500 pg/ml, were demonstrated to specifically recognize at least two different epitopes in gp43 binding assays. Specific antibodies in the sera of patients with active PCM were detected at dilutions of as high as 1:819,200, and the reactivities of patient sera, as measured by optical densities, were found to be significantly higher than those of control sera. The comparison between classical ELISA and our capture enzyme immunoassay showed that both sensitivity and specificity were greatly improved by the latter. These MAbs represent the first specific reagents to P. brasiliensis described for use in serological tests for PCM.
针对巴西副球孢子菌43 kDa糖蛋白产生的四种鼠单克隆抗体（单克隆抗体17C、21A、21F和32B），在捕获酶免疫测定（EIA）中进行了测试，以检测副球孢子菌病（PCM）患者体内特异性人抗gp43免疫球蛋白G。所有单克隆抗体在该测定中反应相似。这些单克隆抗体在低至500 pg/ml的水平就能检测到抗gp43，在gp43结合试验中被证明能特异性识别至少两个不同的表位。在高达1:819,200的稀释度下检测到了活动性PCM患者血清中的特异性抗体，通过光密度测量发现患者血清的反应性显著高于对照血清。经典ELISA与我们的捕获酶免疫测定之间的比较表明，后者的敏感性和特异性都有很大提高。这些单克隆抗体是首次描述用于PCM血清学检测的针对巴西副球孢子菌的特异性试剂。
