Hellema H W, Rümke P
Clin Exp Immunol. 1978 Jan;31(1):12-7.
Human sperm-agglutinating sera was examined using the tray agglutination technique (TAT) with whole diluted semen, and also with a suspension of only progressively motile spermatozoa-obtained from the same semen sample by the penetration of motile spermatozoa in an overlying medium layer. The titres of tail-to-tail (T) agglutinating sera were considerably lower with the latter sperm suspension, but head-to-head (H) agglutinating sera gave similar titres with the two types of sperm suspensions. Attempts to increase titres of T-agglutinating sera with only progressively motile spermatozoa were made by reducing the progression rate of the spermatozoa, and by increasing the concentration of the sperm cells. However, significant titre differences remained. Moreover, the agglutinates obtained were very small, and difficult to observe with the routinely employed magnification of x40. The lower titres still remained where seminal plasma was used as the penetration medium, indicating that a loss of coating antigen(s) was not the cause of lower titres. It seems likely that spermatozoa are attached to each other only weakly in T agglutination, much more weakly than in H agglutination. It is considered that immotile sperm cells that are present in whole diluted semen reinforce the T agglutination process, producing the higher titres.
使用托盘凝集技术（TAT），对全稀释精液以及仅对通过活动精子穿透上层介质层从同一精液样本中获得的进行性活动精子悬液进行人精子凝集血清检测。对于后一种精子悬液，尾对尾（T）凝集血清的效价显著降低，但头对头（H）凝集血清对两种类型的精子悬液的效价相似。通过降低精子的前进速度和增加精子细胞浓度，试图提高仅对进行性活动精子的T凝集血清的效价。然而，效价仍存在显著差异。此外，获得的凝集物非常小，在常规使用的40倍放大倍数下很难观察到。当使用精浆作为穿透介质时，效价仍然较低，这表明包被抗原的丢失不是效价降低的原因。似乎在T凝集中精子彼此之间的附着非常弱，比在H凝集中弱得多。据认为，全稀释精液中存在的不活动精子细胞增强了T凝集过程，产生了更高的效价。
