Russolo S, Sala A, Crotti D
Quad Sclavo Diagn. 1984 Dec;20(4):372-8.
Four different commercial systems were compared to identify 110 medically important yeast-like fungi found out in as many pathologic materials in three Medical Centers. The systems were: a) API 20C Aux (Ayerst), which represented the reference method in conjunction with the germ-tube test, aggregates 19 carbohydrate substrates for assimilation reactions; b) Uni-Yeast Tek (Flow) and Mycotube (Roche) were used to indicate assimilation and fermentation of 7 carbohydrate substrates and to determine other biochemical tests; c) Candida-Check (Poli) was based on agglutination reaction by specific antisera. Comparative testing of the methods provided correct identifications for 99.1% by API 20C Aux, 95.4% by Candida-Check, 89.1% by Mycotube, 88.2% by Uni-Yeast Tek. The good concordance of the methods and their optimal manuality, rapidity, and economy suggest a wider diffusion in microbiological laboratories.
对四种不同的商业系统进行了比较,以鉴定在三个医疗中心的同样多病理材料中发现的110种具有医学重要性的类酵母真菌。这些系统分别是:a) API 20C Aux(艾尔斯特公司),它与芽管试验一起作为参考方法,汇总了19种用于同化反应的碳水化合物底物;b) Uni-Yeast Tek(福罗公司)和Mycotube(罗氏公司)用于检测7种碳水化合物底物的同化和发酵情况,并进行其他生化试验;c) Candida-Check(波利公司)基于特异性抗血清的凝集反应。方法的比较测试结果显示,API 20C Aux的正确鉴定率为99.1%,Candida-Check为95.4%,Mycotube为89.1%,Uni-Yeast Tek为88.2%。这些方法的良好一致性以及它们最佳的手工操作性、快速性和经济性表明,它们在微生物实验室中具有更广泛应用的潜力。