Lin C C, Fung D Y
Department of Quality Assurance, Burger King Corporation, Miami, Florida.
Crit Rev Microbiol. 1987;14(4):273-89. doi: 10.3109/10408418709104441.
Identification of yeasts depends on data obtained from morphological features and physiological characteristics. This article reviews the commonly used conventional methods for the identification of yeast to set the stage for a critical review of rapid systems for yeast identification. Comparative analyses of percentage agreement of API 20C, Uni-Yeast Tek, Minitek, Mycotube, Micro-Drop, Auxodisk, Iatron, Autobac 1, AMS, Abbott MS-2, and Abbott Quantum 11 against the conventional method and, in some cases, against each other are tabulated. Gas liquid chromatography method, enzyme method, MUG, and the Fung's mini-system are also mentioned as possible rapid methods for yeast identification. The purpose of this review is to update the rapidly expanding information concerning diagnostic kits and systems for yeast identification and analyze some of the advantages, disadvantages and potential uses of rapid systems of yeast identification.
酵母的鉴定依赖于从形态特征和生理特性获得的数据。本文回顾了用于酵母鉴定的常用传统方法,为批判性审视酵母鉴定的快速系统奠定基础。文中列表比较分析了API 20C、Uni-Yeast Tek、Minitek、Mycotube、Micro-Drop、Auxodisk、Iatron、Autobac 1、AMS、雅培MS-2和雅培Quantum 11与传统方法以及在某些情况下相互之间的符合率百分比。气液色谱法、酶法、MUG和冯氏微型系统也被提及作为酵母鉴定可能的快速方法。本综述的目的是更新有关酵母鉴定诊断试剂盒和系统的迅速扩展的信息,并分析酵母鉴定快速系统的一些优点、缺点和潜在用途。