Wadlin J K, Hanko G, Stewart R, Pape J, Nachamkin I
Clinical Microbiology Laboratory, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104-4283, USA.
J Clin Microbiol. 1999 Jun;37(6):1967-70. doi: 10.1128/JCM.37.6.1967-1970.1999.
We evaluated three commercial systems (RapID Yeast Plus System; Innovative Diagnostic Systems, Norcross, Ga.; API 20C Aux; bioMerieux-Vitek, Hazelwood, Mo.; and Vitek Yeast Biochemical Card, bioMerieux-Vitek) against an auxinographic and microscopic morphologic reference method for the ability to identify yeasts commonly isolated in our clinical microbiology laboratory. Two-hundred one yeast isolates were compared in the study. The RapID Yeast Plus System was significantly better than either API 20C Aux (193 versus 167 correct identifications; P < 0.0001) or the Vitek Yeast Biochemical Card (193 versus 173 correct identifications; P = 0.003) for obtaining correct identifications to the species level without additional testing. There was no significant difference between results obtained with API 20C Aux and the Vitek Yeast Biochemical Card system (P = 0.39). The API 20C Aux system did not correctly identify any of the Candida krusei isolates (n = 23) without supplemental testing and accounted for the major differences between the API 20C Aux and RapID Yeast Plus systems. Overall, the RapID Yeast Plus System was easy to use and is a good system for the routine identification of clinically relevant yeasts.
我们针对一种生长素培养成像和显微镜形态学参考方法，评估了三种商业系统（RapID酵母增强系统；创新诊断系统公司，佐治亚州诺克罗斯市；API 20C Aux；生物梅里埃-维泰克公司，密苏里州黑兹尔伍德市；以及维泰克酵母生化鉴定卡，生物梅里埃-维泰克公司）识别我们临床微生物实验室常见分离酵母的能力。本研究中比较了201株酵母分离株。在无需额外检测就能获得种水平的正确鉴定方面，RapID酵母增强系统显著优于API 20C Aux（正确鉴定数分别为193和167；P<0.0001）或维泰克酵母生化鉴定卡（正确鉴定数分别为193和173；P = 0.003）。API 20C Aux和维泰克酵母生化鉴定卡系统的结果之间没有显著差异（P = 0.39）。未经补充检测时，API 20C Aux系统未能正确鉴定任何一株克柔念珠菌分离株（n = 23），这也是API 20C Aux和RapID酵母增强系统之间存在主要差异的原因。总体而言，RapID酵母增强系统易于使用，是临床相关酵母常规鉴定的良好系统。
