Schönenbrücher Vanessa, Mallinson Edward T, Bülte Michael
Justus Liebig University of Giessen, Faculty of Veterinary Medicine, Institute of Veterinary Food Science, Frankfurter Str. 92, D-35392 Giessen, Germany.
Int J Food Microbiol. 2008 Mar 31;123(1-2):61-6. doi: 10.1016/j.ijfoodmicro.2007.11.064. Epub 2007 Dec 4.
In this study the draft of the horizontal method for the detection of Salmonella species from human food and animal feed (ISO 6579:2002) was compared to the European gold standard (DIN EN 12824:1998), including the three new chromogenic plating media AES Salmonella Agar Plate (ASAP), Oxoid Salmonella Chromogen Media (OSCM) and Miller-Mallinson agar (MM). First the growth and appearance of 36 bacterial type strains (Salmonella and other 21 species) on ASAP, OSCM and MM were compared to those on the three traditional agars Brilliant Green Agar according to Edel and Kampelmacher (BGA), Xylose Lysine Deoxycholate Agar (XLD) and Xylose Lysine Tergitol 4 Agar (XLT4). Only on MM agar, did all of 36 tested type strains produce typical colonies, especially strains of S. Senftenberg, Salmonella arizonae, S. Dublin and S. Derby. Artificial inoculation experiments using raw pork ground meat (n=92) were subsequently conducted. A shortened incubation time of 24 h in RVS broth yielded a Salmonella species recovery of 100% from spiked meat samples. Finally, 286 naturally contaminated raw porcine and bovine minced meat samples and raw poultry meat samples were investigated. Forty-three strains from a total of 39 Salmonella-positive samples were found. S. Typhimurium (n=21), with DT 104 L, DT 012 and RDNC being the most prevalent subtypes isolated. D-tartrate-positive S. Paratyphi B (n=2) and S. Saint-Paul (n=3) were also recovered. They were cultured from poultry meat and were multi-resistant against antibiotics including nalidixic acid. Rappaport Vassiliadis broth with soypeptone (RVS) yielded the highest recovery of Salmonella spp. (97,4%) compared to Tetrathionate broth with Novobiocin according to Muller and Kauffman (MKTTn, 94,9%) and Selenite Cystine broth (SC, 38,5%). However, no significant difference was obtained by comparing the ISO 6579:2002 draft to the gold standard.
在本研究中，将用于检测人类食品和动物饲料中沙门氏菌属的水平方法草案（ISO 6579:2002）与欧洲金标准（DIN EN 12824:1998）进行了比较，其中包括三种新型显色平板培养基，即AES沙门氏菌琼脂平板（ASAP）、Oxoid沙门氏菌显色培养基（OSCM）和Miller-Mallinson琼脂（MM）。首先，将36种细菌标准菌株（沙门氏菌和其他21个菌种）在ASAP、OSCM和MM上的生长情况及菌落形态与在三种传统琼脂培养基上的情况进行比较，这三种传统琼脂培养基分别是根据Edel和Kampelmacher方法制备的亮绿琼脂（BGA）、木糖赖氨酸脱氧胆酸盐琼脂（XLD）和木糖赖氨酸吐温4琼脂（XLT4）。仅在MM琼脂上，36种受试标准菌株均产生了典型菌落，尤其是肠炎沙门氏菌、亚利桑那沙门氏菌、都柏林沙门氏菌和德比沙门氏菌的菌株。随后进行了使用生猪肉末（n = 92）的人工接种实验。在RVS肉汤中缩短至24小时的培养时间，使加标肉样中沙门氏菌属的回收率达到100%。最后，对286份天然污染的生猪和牛绞肉样品以及生禽肉样品进行了调查。在总共39份沙门氏菌阳性样品中发现了43株菌株。鼠伤寒沙门氏菌（n = 21），其中DT 104 L、DT 012和RDNC是分离出的最常见亚型。还分离出了D-酒石酸盐阳性的副伤寒乙沙门氏菌（n = 2）和圣保罗沙门氏菌（n = 3）。它们是从禽肉中培养出来的，并且对包括萘啶酸在内的多种抗生素具有多重耐药性。与根据Muller和Kauffman方法制备的含新生霉素的四硫磺酸盐肉汤（MKTTn，94.9%）和亚硒酸盐胱氨酸肉汤（SC，38.5%）相比，含胰蛋白胨的Rappaport Vassiliadis肉汤（RVS）对沙门氏菌属的回收率最高（97.4%）。然而，将ISO 6579:2002草案与金标准进行比较时，未获得显著差异。
