Tenenbaum S, DiNardo J, Morris W E, Wolf B A, Schnetzinger R W
Revlon Research Center, Inc., Edison, NJ 08818.
Cell Biol Toxicol. 1984 Oct;1(1):1-9. doi: 10.1007/BF00125560.
A quantitative in vitro method for phototoxic evaluation of chemicals has been developed and validated. The assay uses Saccharomyces cerevisiae, seeded in an agar overlay on top of a plate count agar base. 8-Methoxy psoralen is used as a reference standard against which materials are measured. Activity is quantified by cytotoxicity measured as zones of inhibition. Several known phototoxins (heliotropine, lyral, phantolid, and bergamot oil) and photoallergens (6-methyl coumarin and musk ambrette) are used to validate the assay. An excellent correlation is observed between in vivo studies employing Hartley albino guinea pigs and the in vitro assay for several fragrance raw materials and other chemicals. The in vitro assay exhibits a greater sensitivity from 2-500 fold. For three fragrance oils, the in vitro assay detects low levels of photobiological activity while the in vivo assay is negative. Although the in vitro assay does not discriminate between phototoxins and photoallergens, it can be used for screening of raw materials so that reduction in animal usage can be achieved while maintaining the protection of the consumer.
一种用于化学品光毒性评估的定量体外方法已被开发并验证。该测定法使用酿酒酵母，接种在平板计数琼脂基础上的琼脂覆盖层中。8-甲氧基补骨脂素用作参考标准，据此对材料进行测量。活性通过以抑制圈衡量的细胞毒性进行量化。几种已知的光毒素（天芥菜精、莱雅、幻吲哚和佛手柑油）和光变应原（6-甲基香豆素和葵子麝香）用于验证该测定法。在使用哈特利白化豚鼠的体内研究与针对几种香料原料和其他化学品的体外测定法之间观察到了极好的相关性。体外测定法的灵敏度提高了2至500倍。对于三种香料油，体外测定法检测到低水平的光生物活性，而体内测定法为阴性。尽管体外测定法无法区分光毒素和光变应原，但它可用于原料筛选，从而在保护消费者的同时减少动物使用量。
