宿主对肺炎支原体蛋白质免疫原的识别。
Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens.
作者信息
Leith D K, Trevino L B, Tully J G, Senterfit L B, Baseman J B
出版信息
J Exp Med. 1983 Feb 1;157(2):502-14. doi: 10.1084/jem.157.2.502.
Abstract
The immune response of experimentally infected hamsters and human patients to Mycoplasma pneumoniae was examined by radioimmunoprecipation in conjunction with gel electrophoresis and fluorography. Both intrinsically and extrinsically labeled mycoplasma proteins were coincubated with acute and convalescent sera in a radioimmunoprecipitation assay. Two M. pneumoniae proteins were selectively precipitated by convalescent sera. These predominant immunogens were trypsin-sensitive, antibody-accessible surface proteins that co-migrate on polyacrylamide gels with proteins P1 and P2, which were previously implicated by us as mediators of cytadsorption. Anti-M. pneumoniae antiserum did not precipitate radiolabeled antigens derived from Mycoplasma orale or Mycoplasma salivarium. These data indicate that M. pneumoniae infection stimulates a specific and highly targeted host antibody response to key proteinaceous immunogens.
摘要
通过放射免疫沉淀结合凝胶电泳和荧光自显影技术，检测了实验感染的仓鼠和人类患者对肺炎支原体的免疫反应。在放射免疫沉淀试验中，将内在和外在标记的支原体蛋白与急性期和恢复期血清共同孵育。恢复期血清选择性沉淀出两种肺炎支原体蛋白。这些主要的免疫原是对胰蛋白酶敏感、抗体可及的表面蛋白，它们在聚丙烯酰胺凝胶上与蛋白P1和P2共同迁移，我们之前认为P1和P2是细胞吸附的介质。抗肺炎支原体抗血清不会沉淀来自口腔支原体或唾液支原体的放射性标记抗原。这些数据表明，肺炎支原体感染会刺激宿主对关键蛋白质免疫原产生特异性且高度靶向的抗体反应。
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