Jagger K S, Bahner D R, Warren R L
J Clin Microbiol. 1983 Jan;17(1):55-9. doi: 10.1128/jcm.17.1.55-59.1983.
The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expression of protease activity, although a higher percentage of nonmucoid isolates was proteolytic. When isolates from CF patients chronically infected with P. aeruginosa were compared to isolates from CF patients colonized with this organism, both groups were found to contain comparable percentages of elastase-producing strains and mucoid strains. However, the group of isolates from colonized patients contained a higher percentage of strains producing alkaline protease and expressing general protease activity. In addition, the group of isolates from chronically infected patients contained more weakly proteolytic isolates than either the group from colonized CF patients or a group of isolates from pediatric patients without CF. These data suggest that protease production may be important in the initial colonization of the respiratory tract of CF patients by P. aeruginosa.
对从囊性纤维化（CF）患者中分离出的铜绿假单胞菌菌株所表达的蛋白酶表型进行了评估。大多数测试菌株产生弹性蛋白酶（65%）或碱性蛋白酶（64%）或两者皆有。许多CF患者的铜绿假单胞菌黏液型菌株虽然产生蛋白酶活性的非黏液型菌株比例更高，但并不绝对限制蛋白酶活性的表达。将长期感染铜绿假单胞菌的CF患者分离株与定植有该菌的CF患者分离株进行比较时，发现两组中产生弹性蛋白酶的菌株和黏液型菌株的比例相当。然而，定植患者的分离株组中产生碱性蛋白酶并表达一般蛋白酶活性的菌株比例更高。此外，长期感染患者的分离株组中弱蛋白水解性分离株比定植CF患者组或无CF的儿科患者分离株组都更多。这些数据表明，蛋白酶的产生可能在铜绿假单胞菌对CF患者呼吸道的初始定植中起重要作用。
