Janda J M, Bottone E J
J Clin Microbiol. 1981 Jul;14(1):55-60. doi: 10.1128/jcm.14.1.55-60.1981.
The enzymatic potential of 54 clinical and 22 environmental isolates of Pseudomonas aeruginosa from soil and water were evaluated by substrate plate assays. Clinical isolates produced substantial levels of 9 of the 11 enzymes assayed, whereas strains recovered from soil or water were relatively inert enzymatically. Elastase, deoxyribonuclease, and elevated protease activities were associated preferentially with clinical isolates of systemic origin; these activities were found twice as frequently in clinical isolates as in strains derived from sputum or the urogenital tract. Our data suggest that these factors may play an important role in the dissemination of P. aeruginosa from local or superficial sites. A comparison of the enzyme profiles of the environmental and clinical isolates indicated that colonization or infection by environmental strains of P. aeruginosa is a rare event and that environmental and clinical strains comprise separate biovars. Epidemiologically, enzyme profiles permitted the fingerprinting and differentiation of clinical strains from various sources.
通过底物平板试验评估了从土壤和水中分离出的54株临床铜绿假单胞菌菌株以及22株环境铜绿假单胞菌菌株的酶活性潜力。临床分离株在所检测的11种酶中有9种产生了大量的酶，而从土壤或水中分离出的菌株在酶活性方面相对不活跃。弹性蛋白酶、脱氧核糖核酸酶和较高的蛋白酶活性优先与系统性来源的临床分离株相关；这些活性在临床分离株中的出现频率是痰或泌尿生殖道来源菌株的两倍。我们的数据表明，这些因素可能在铜绿假单胞菌从局部或浅表部位的传播中起重要作用。对环境分离株和临床分离株的酶谱进行比较表明，环境铜绿假单胞菌菌株的定植或感染是罕见事件，并且环境菌株和临床菌株构成不同的生物变种。从流行病学角度来看，酶谱允许对来自不同来源的临床菌株进行指纹识别和区分。
