Thoen C O, Bruner J A, Luchsinger D W, Pietz D E
Am J Vet Res. 1983 Feb;44(2):306-8.
Enzyme-linked immunosorbent assays were conducted on milk of cows from which Brucella abortus was isolated and that of noninfected controls. Horseradish peroxidase-labeled rabbit antibovine immunoglobulins IgG, IgG1, and IgA were used as conjugates. A heat-killed whole-cell suspension of B abortus strain 19 was used as the antigen. Differences in antibody profiles were observed in milk of cows from which B abortus was isolated and in milk of noninfected cows. Antibody profiles were similar in milk of cows infected with B abortus and that of cows from which B abortus strain 19 was isolated.
对分离出流产布鲁氏菌的奶牛的牛奶以及未感染的对照奶牛的牛奶进行了酶联免疫吸附测定。用辣根过氧化物酶标记的兔抗牛免疫球蛋白IgG、IgG1和IgA作为结合物。用流产布鲁氏菌19株的热灭活全细胞悬液作为抗原。在分离出流产布鲁氏菌的奶牛的牛奶和未感染奶牛的牛奶中观察到抗体谱的差异。感染流产布鲁氏菌的奶牛的牛奶和分离出流产布鲁氏菌19株的奶牛的牛奶中的抗体谱相似。
