Miura K, Tsuda S, Ueda T, Harada F, Kato N
Biochim Biophys Acta. 1983 Apr 15;739(3):281-5. doi: 10.1016/0167-4781(83)90102-1.
Chemical modification of mouse 5 S rRNA with kethoxal was carried out to examine the secondary structure. The guanine residues located at positions 37, 41, 56, 66, 75 and 89 were modified. The relative rates of reaction are in the order G37, G56, G89, G66, G41, G75 at 28 degrees C and G37, G41, G56, G89, G75, G66 at 35 degrees C. These results support a secondary structure model containing 5 helices and 5 loops and indicate that the region around position 37 is the most exposed in higher-order structure.
用乙二醛对小鼠5S rRNA进行化学修饰以检测其二级结构。位于37、41、56、66、75和89位的鸟嘌呤残基被修饰。在28℃时反应的相对速率顺序为G37、G56、G89、G66、G41、G75，在35℃时为G37、G41、G56、G89、G75、G66。这些结果支持了一个包含5个螺旋和5个环的二级结构模型，并表明37位附近的区域在高级结构中最暴露。
