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使用布鲁氏菌多糖抗原的径向免疫扩散试验，用于区分感染牛和接种疫苗的牛。

Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle.

作者信息

Diaz R, Garatea P, Jones L M, Moriyon I

出版信息

J Clin Microbiol. 1979 Jul;10(1):37-41. doi: 10.1128/jcm.10.1.37-41.1979.

DOI:10.1128/jcm.10.1.37-41.1979
PMID:115896
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273089/
Abstract

A Brucella antigen containing polysaccharide but lacking smooth lipopolysaccharide was employed in a rapid radial immunodiffusion test. With this serological test, cattle infected with Brucella abortus could be identified in recently vaccinated herds which had high numbers of reactors to standard diagnostic tests.

摘要

一种含有多糖但缺乏光滑脂多糖的布鲁氏菌抗原被用于快速单向免疫扩散试验。通过这种血清学检测，在近期接种过疫苗且对标准诊断检测有大量反应动物的牛群中，可以识别出感染了流产布鲁氏菌的牛。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/857a3c205e70/jcm00180-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/4f4dfec86dd2/jcm00180-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/43293ef3d646/jcm00180-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/857a3c205e70/jcm00180-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/4f4dfec86dd2/jcm00180-0056-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/43293ef3d646/jcm00180-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f11/273089/857a3c205e70/jcm00180-0057-b.jpg

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Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle.使用布鲁氏菌多糖抗原的径向免疫扩散试验，用于区分感染牛和接种疫苗的牛。
J Clin Microbiol. 1979 Jul;10(1):37-41. doi: 10.1128/jcm.10.1.37-41.1979.
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本文引用的文献

1
Some structural and biological properties of Brucella endotoxin.布鲁氏菌内毒素的一些结构和生物学特性。
Infect Immun. 1970 Feb;1(2):174-82. doi: 10.1128/iai.1.2.174-182.1970.
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[Contribution to the serological diagnosis of brucellosis and yersiniosis. I. Use of the gel precipitation reaction].
Rev Clin Esp. 1971 May;121(4):367-72.
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[Respective role in bovine Brucellosis serology of antigens and immunoglobulins G 1 and G 2 in the Coombs and Rose Bengal agglutination tests and also in the zone phenomenon].[抗原以及免疫球蛋白G1和G2在库姆斯试验和玫瑰红平板凝集试验中对牛布鲁氏菌病血清学的各自作用以及在带现象中的作用]
接种布鲁氏菌疫苗但血清呈阳性的荷斯坦奶牛的产奶量和繁殖性能。
Trop Anim Health Prod. 2014 Feb;46(2):391-7. doi: 10.1007/s11250-013-0502-4. Epub 2013 Nov 20.
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Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1.布鲁氏菌属 melitensis 地方性菌株的分子分型与疫苗菌株 Rev-1 的区分。
Vet Res Commun. 2012 Mar;36(1):7-20. doi: 10.1007/s11259-011-9505-7. Epub 2011 Oct 22.
5
Use of the Brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay.利用布鲁氏菌天然半抗原通过快速、简单且特异的荧光偏振分析法诊断山羊布鲁氏菌病。
Clin Vaccine Immunol. 2008 Jun;15(6):911-5. doi: 10.1128/CVI.00046-08. Epub 2008 Apr 2.
6
The lipopolysaccharide of Brucella abortus BvrS/BvrR mutants contains lipid A modifications and has higher affinity for bactericidal cationic peptides.流产布鲁氏菌BvrS/BvrR突变体的脂多糖含有脂质A修饰，且对杀菌阳离子肽具有更高的亲和力。
J Bacteriol. 2005 Aug;187(16):5631-9. doi: 10.1128/JB.187.16.5631-5639.2005.
7
Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9.在存在因小肠结肠炎耶尔森菌O:9导致的血清学假阳性结果的情况下，几种血清学检测方法和抗原对牛布鲁氏菌病诊断的效能
Clin Diagn Lab Immunol. 2005 Jan;12(1):141-51. doi: 10.1128/CDLI.12.1.141-151.2005.
8
Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.用流产布鲁氏菌S19疫苗接种或经小肠结肠炎耶尔森菌0:9血清型实验感染的牛，针对流产布鲁氏菌脂多糖和细胞质蛋白的体液免疫反应
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9
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J Bacteriol. 1996 Feb;178(4):1070-9. doi: 10.1128/jb.178.4.1070-1079.1996.
10
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J Clin Microbiol. 1993 Dec;31(12):3136-41. doi: 10.1128/jcm.31.12.3136-3141.1993.
C R Acad Hebd Seances Acad Sci D. 1972 Mar 6;274(10):1593-6.
4
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5
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J Clin Microbiol. 1976 Feb;3(2):203-5. doi: 10.1128/jcm.3.2.203-205.1976.
6
Comparison of counter-immunoelectrophoresis with other serological tests in the diagnosis of human brucellosis.对流免疫电泳与其他血清学检测方法在人类布鲁氏菌病诊断中的比较。
Bull World Health Organ. 1976;53(4):417-24.
7
Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus.流产布鲁氏菌光滑型和粗糙型脂多糖的纯化与特性分析
J Bacteriol. 1979 May;138(2):361-9. doi: 10.1128/jb.138.2.361-369.1979.