Koch E A, Spitzer R H
Cell Tissue Res. 1983;228(1):21-32. doi: 10.1007/BF00206261.
Adult female fruitflies exposed to colchicine admixed to the culture medium show a series of dosage-related abnormalities that affect oogenesis and may induce sterility. Among the effects observed were decreased fecundity and hatchability of laid eggs, formation of oocytes lacking chorionic appendages, abnormal distribution and diminution in number of yolk spheres, inhibition of oocyte growth and abnormally located oocyte nuclei. Potentially the most significant effect was the development of egg chambers which contained the normal complement of 16 cells but in which all the cells had the nuclear morphology of nurse cells. The approach provides for the first time an experimental means to divert a potential oocyte into the developmental pathway of the nurse cell in a wild-type fly, and hence should be helpful in the elucidation of factors which control oocyte and nurse cell differentiation. In addition, the results serve to expand the usefulness of oogenesis in Drosophila as a model system for the evaluation of drug-induced metabolic-morphologic abnormalities.
暴露于添加秋水仙碱的培养基中的成年雌性果蝇会出现一系列与剂量相关的异常情况，这些异常会影响卵子发生并可能导致不育。观察到的影响包括产卵量和孵化率降低、缺乏绒毛附属物的卵母细胞形成、卵黄球分布异常和数量减少、卵母细胞生长受抑制以及卵母细胞核位置异常。最显著的潜在影响是卵室的发育，其中包含正常的16个细胞，但所有细胞都具有滋养细胞的核形态。该方法首次提供了一种实验手段，可使野生型果蝇中的潜在卵母细胞转向滋养细胞的发育途径，因此有助于阐明控制卵母细胞和滋养细胞分化的因素。此外，这些结果有助于扩大果蝇卵子发生作为评估药物诱导的代谢形态异常的模型系统的用途。
