Kobayashi K, Shiino M
Cell Tissue Res. 1983;229(1):197-205. doi: 10.1007/BF00217892.
Gonadotrophin release from a monolayer of cultured pituitary cells from the rat was increased by the addition of fresh rat serum or rat serum albumin to the culture medium. Similar effects were obtained when horse, chicken, lamb or porcine serum was used. Fetal bovine serum or bovine serum albumin showed a stimulatory effect on FSH and LH release but, unlike the case with rat serum, a dose-response release could not be demonstrated. In addition, fresh rat serum caused the accumulation of secretory granules in the gonadotrophs. The addition of LHRH to the culture medium also stimulated gonadotrophin release in a dose-response dependent manner. The dependence of the gonadotrophs on LHRH was markedly increased by the addition of 50% fresh rat serum to the culture medium. These results indicate that fresh rat serum contains some unknown factor(s) which 1) stimulates gonadotrophin release, 2) causes an accumulation of secretory granules, and 3) is important for maintaining secretory capacity of gonadotrophs.
向培养基中添加新鲜大鼠血清或大鼠血清白蛋白后，来自大鼠的单层培养垂体细胞释放的促性腺激素增加。使用马、鸡、羊或猪血清时也获得了类似的效果。胎牛血清或牛血清白蛋白对促卵泡激素（FSH）和促黄体生成素（LH）的释放有刺激作用，但与大鼠血清不同，无法证明其存在剂量反应性释放。此外，新鲜大鼠血清导致促性腺细胞中分泌颗粒的积累。向培养基中添加促性腺激素释放激素（LHRH）也以剂量反应依赖的方式刺激促性腺激素释放。向培养基中添加50%新鲜大鼠血清可显著增强促性腺细胞对LHRH的依赖性。这些结果表明，新鲜大鼠血清含有一些未知因子，这些因子1）刺激促性腺激素释放，2）导致分泌颗粒积累，3）对维持促性腺细胞的分泌能力很重要。
