Weiblen B J, Melaragno A J, Catsimpoolas N, Valeri C R
J Immunol Methods. 1983 Mar 11;58(1-2):73-81. doi: 10.1016/0022-1759(83)90264-8.
The distribution of radioactivity on plasma proteins labeled by addition of [111In]oxine to citrated plasma was investigated. Analyses of plasma proteins separated on Sephadex G-200 columns showed that 23-36% of the 111In was associated with proteins with molecular weight greater than 200,000 daltons and the remaining 111In was associated with proteins with molecular weight less than 100,000 daltons, presumably transferrin. Affinity chromatography experiments showed that less than 2% of the radioactivity was associated with albumin. Further identification of the labeled proteins and quantitation of associated radioactivity was performed by precipitating specific proteins with antibodies. These studies showed that the 111In was distributed on transferrin (54-76%), fibrinogen (11-24%), IgM (8-20%), C3 (10-21%), and haptoglobin (3-8%). 111In associated with fibrinogen, IgM, and haptoglobin was over-estimated in some experiments due to binding of 111In-labeled C3 to the antigen-antibody precipitates.
研究了在枸橼酸盐血浆中加入[111铟]氧肟酸后放射性在血浆蛋白上的分布情况。对在葡聚糖凝胶G - 200柱上分离的血浆蛋白进行分析表明，111铟中有23 - 36%与分子量大于200,000道尔顿的蛋白质结合，其余的111铟与分子量小于100,000道尔顿的蛋白质结合，推测为转铁蛋白。亲和层析实验表明，放射性与白蛋白结合的比例不到2%。通过用抗体沉淀特定蛋白质对标记蛋白质进行进一步鉴定和对相关放射性进行定量。这些研究表明，111铟分布在转铁蛋白（54 - 76%）、纤维蛋白原（11 - 24%）、IgM（8 - 20%）、C3（10 - 21%）和触珠蛋白（3 - 8%）上。在一些实验中，由于111铟标记的C3与抗原 - 抗体沉淀物结合，与纤维蛋白原、IgM和触珠蛋白结合的111铟被高估了。
