使用两种检测方法改进结核病的血清学诊断。
Improved serodiagnosis of tuberculosis using two assay test.
作者信息
Krambovitis E, Harris M, Hughes D T
出版信息
J Clin Pathol. 1986 Jul;39(7):779-85. doi: 10.1136/jcp.39.7.779.
Abstract
An antigen capture immunoassay was developed for the detection of mycobacterial antigens in sera from patients with tuberculosis. The assay was evaluated together with an antibody measuring enzyme immunoassay in a clinical trial for serodiagnosis of tuberculosis. Sensitivity of the antibody assay for active pulmonary tuberculosis, including relapsed infections, was 75%, and specificity with other lung diseases was 97%. Sensitivity for extrapulmonary tuberculosis was 84.5% and specificity 84%. Sensitivity of the antigen assay for active tuberculosis was 45% with no false positive reactions. Combination of the results from the two assays increased total sensitivity to 96.5% with a positive predictive value of 0.81 and a negative value of 0.98. The two assay test was relatively simple to perform and offered improved serological diagnosis of tuberculosis over a single antibody test.
摘要
开发了一种抗原捕获免疫测定法，用于检测结核病患者血清中的分枝杆菌抗原。在一项结核病血清诊断临床试验中，该测定法与一种抗体检测酶免疫测定法一起进行了评估。抗体测定法对包括复发性感染在内的活动性肺结核的敏感性为75%，对其他肺部疾病的特异性为97%。肺外结核的敏感性为84.5%，特异性为84%。抗原测定法对活动性结核的敏感性为45%，无假阳性反应。两种测定法结果的组合使总敏感性提高到96.5%，阳性预测值为0.81，阴性预测值为0.98。两种测定法检测相对简单，与单一抗体检测相比，可改善结核病的血清学诊断。
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