Krambovitis E, Harris M, Hughes D T
J Clin Pathol. 1986 Jul;39(7):779-85. doi: 10.1136/jcp.39.7.779.
An antigen capture immunoassay was developed for the detection of mycobacterial antigens in sera from patients with tuberculosis. The assay was evaluated together with an antibody measuring enzyme immunoassay in a clinical trial for serodiagnosis of tuberculosis. Sensitivity of the antibody assay for active pulmonary tuberculosis, including relapsed infections, was 75%, and specificity with other lung diseases was 97%. Sensitivity for extrapulmonary tuberculosis was 84.5% and specificity 84%. Sensitivity of the antigen assay for active tuberculosis was 45% with no false positive reactions. Combination of the results from the two assays increased total sensitivity to 96.5% with a positive predictive value of 0.81 and a negative value of 0.98. The two assay test was relatively simple to perform and offered improved serological diagnosis of tuberculosis over a single antibody test.
开发了一种抗原捕获免疫测定法，用于检测结核病患者血清中的分枝杆菌抗原。在一项结核病血清诊断临床试验中，该测定法与一种抗体检测酶免疫测定法一起进行了评估。抗体测定法对包括复发性感染在内的活动性肺结核的敏感性为75%，对其他肺部疾病的特异性为97%。肺外结核的敏感性为84.5%，特异性为84%。抗原测定法对活动性结核的敏感性为45%，无假阳性反应。两种测定法结果的组合使总敏感性提高到96.5%，阳性预测值为0.81，阴性预测值为0.98。两种测定法检测相对简单，与单一抗体检测相比，可改善结核病的血清学诊断。
