Croker B P, Kuhn R E
Histochemistry. 1983;77(2):195-200. doi: 10.1007/BF00506562.
We compared two immunoperoxidase procedures for the identification of Trypanosoma cruzi antigens in wax sections of infected mouse tissues. Both an indirect immunoperoxidase and an avidin-biotinated horseradish peroxidase complex (ABC) procedure gave identical patterns of localization. The ABC procedure could be used with greater dilutions of antisera than the indirect procedure. Individual organisms were clearly identified in the absence of inflammation at scanning magnifications. In addition, fragments of parasites were identified in inflammatory lesions. Therefore, this technique should be valuable in experimental and clinical situations when the identification of few organisms or fragments of organisms is required.
我们比较了两种免疫过氧化物酶方法，用于在感染小鼠组织的石蜡切片中鉴定克氏锥虫抗原。间接免疫过氧化物酶法和抗生物素蛋白-生物素化辣根过氧化物酶复合物（ABC）法给出了相同的定位模式。与间接法相比，ABC法可以使用更高稀释度的抗血清。在扫描放大倍数下，在无炎症的情况下可清晰识别单个生物体。此外，在炎性病变中鉴定出了寄生虫碎片。因此，当需要鉴定少量生物体或生物体碎片时，该技术在实验和临床情况下应具有重要价值。
