Borowitz M J, Croker B P, Metzgar R S
Am J Pathol. 1981 Nov;105(2):97-106.
The lymphocyte surface marker phenotype in 11 selected cases of non-Hodgkin's lymphomas was determined with anti-immunoglobulin and mouse monoclonal antibodies against human lymphocyte antigens. A complement-mediated cell cytotoxicity assay on suspensions of the tumor cells was compared with an indirect immunoperoxidase technique on frozen tissue sections. Both methods gave good results in tumors with a uniform cell population, but the frozen section technique was superior in heterogeneous tumors. The six B-cell neoplasms were heterogeneous with respect to expression of surface immunoglobulin and Ia antigens. The five T-cell tumors were morphologically heterogeneous and also highly variable in their expression of different T-cell specific antigens.
采用抗免疫球蛋白及抗人淋巴细胞抗原的小鼠单克隆抗体，对11例经挑选的非霍奇金淋巴瘤患者的淋巴细胞表面标志物表型进行了测定。将肿瘤细胞悬液的补体介导细胞毒性试验与冷冻组织切片的间接免疫过氧化物酶技术进行了比较。对于细胞群体均匀的肿瘤，两种方法均取得了良好的结果，但冷冻切片技术在异质性肿瘤中表现更优。6例B细胞肿瘤在表面免疫球蛋白和Ia抗原的表达方面具有异质性。5例T细胞肿瘤在形态上具有异质性，并且在不同T细胞特异性抗原的表达上也高度可变。
