Hechemy K E, Anacker R L, Carlo N L, Fox J A, Gaafar H A
J Clin Microbiol. 1983 Mar;17(3):445-9. doi: 10.1128/jcm.17.3.445-449.1983.
Immunoabsorption profiles were determined for Rickettsia rickettsii antigens used in four confirmatory tests for detection of antibodies to Rocky Mountain spotted fever. A human serum reactive in the four tests was absorbed with each test antigen and then reexamined by all four tests. The results indicated that the whole organism and complement-fixation antigen absorbed the whole array of antibodies to R. rickettsii, whereas erythrocyte-sensitizing substance coated on latex or erythrocytes did not. With erythrocyte-sensitizing substance coated on latex there was a decrease in titer when tested by the latex, indirect hemagglutination, and complement fixation tests but none when tested by the microimmunofluorescence test. With erythrocyte-sensitizing substance coated on sheep erythrocytes there was a decrease in titer when tested by indirect hemagglutination or complement fixation but none when tested by latex or microimmunofluorescence. These findings confirm that erythrocyte-sensitizing substance contains more than a single antigenic substance and plays a major role in the complement fixation test and a minor role in the microimmunofluorescence test.
针对用于落基山斑疹热抗体检测的四项确证试验中所使用的立氏立克次体抗原，测定了免疫吸附谱。用每种试验抗原对在这四项试验中呈反应性的一份人血清进行吸附，然后通过所有四项试验重新检测。结果表明，全菌体和补体结合抗原吸附了对立氏立克次体的全部抗体，而包被在乳胶或红细胞上的红细胞致敏物质则没有。对于包被在乳胶上的红细胞致敏物质，通过乳胶、间接血凝和补体结合试验检测时滴度降低，但通过微量免疫荧光试验检测时则无变化。对于包被在绵羊红细胞上的红细胞致敏物质，通过间接血凝或补体结合试验检测时滴度降低，但通过乳胶或微量免疫荧光试验检测时则无变化。这些发现证实，红细胞致敏物质包含不止一种抗原物质，在补体结合试验中起主要作用，而在微量免疫荧光试验中起次要作用。
