采用高密度颗粒凝集法快速检测伯氏考克斯氏体抗体的方法
Rapid method for detection of Coxiella burnetii antibodies using high-density particle agglutination.
作者信息
Nguyen S V, Otsuka H, Zhang G Q, To H, Yamaguchi T, Fukushi H, Noma A, Hirai K
机构信息
Department of Veterinary Microbiology, Gifu University, Japan.
出版信息
J Clin Microbiol. 1996 Dec;34(12):2947-51. doi: 10.1128/jcm.34.12.2947-2951.1996.
A high-density particle agglutination test, using erythrocyte-sensitizing substance from phase II Coxiella burnetii adsorbed to high-density composite particles, was developed for rapid serodiagnosis of Q fever. The test was compared with the microimmunofluorescence test for sensitivity and specificity by using 3,036 human serum samples collected in Gifu Prefecture, Japan. An excellent agreement was found between the two tests for the acute-phase group and paired serum samples, but some discordant results were observed in the single-sample group. The sensitivity and specificity of the high-density particle agglutination test were both 100% in the former group and 81.6 and 99.9%, respectively, in the latter group. The test is a very promising tool for routine serodiagnosis of Q fever because of its simplicity, sensitivity, and specificity.
开发了一种高密度颗粒凝集试验，该试验使用吸附于高密度复合颗粒上的II期伯纳特柯克斯体的红细胞致敏物质，用于Q热的快速血清学诊断。通过使用在日本岐阜县收集的3036份人类血清样本，将该试验与微量免疫荧光试验进行了敏感性和特异性比较。在急性期组和配对血清样本的两种试验之间发现了极好的一致性，但在单样本组中观察到了一些不一致的结果。在前一组中，高密度颗粒凝集试验的敏感性和特异性均为100%，在后一组中分别为81.6%和99.9%。由于其简单性、敏感性和特异性，该试验是Q热常规血清学诊断的一个非常有前景的工具。
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