Shirai A, Dietel J W, Osterman J V
J Clin Microbiol. 1975 Nov;2(5):430-7. doi: 10.1128/jcm.2.5.430-437.1975.
An indirect hemagglutination (IHA) test is described that uses glutaraldehyde-stabilized erythrocytes treated with a rickettsial erythrocyte-sensitizing substance obtained from Rickettsia typhi or Rickettsia rickettsii. The serological reagent was stable for at least 3 months at room temperature and 6 months at 4 C. It exhibited group specificity and no group cross-reactivity. At a minimum dilution of 1:40, acute and early convalescent epidemic and murine typhus antisera showed 86% positive reactors, whereas similar spotted fever antisera had 74% positive reactors. In comparison with the indirect fluorescent antibody test, the IHA procedure gave lower titers but showed comparable detection of seroconversion with most paired sera. The IHA test demonstrated significantly higher titers than the complement fixation test and was more sensitive than either the complement fixation or Weil-Felix test in identifying seroconversion. No agglutination was observed when sensitized erythrocytes were tested with rodent sera known to contain rickettsial antibodies.
本文描述了一种间接血凝（IHA）试验，该试验使用经戊二醛稳定的红细胞，这些红细胞用从鼠型斑疹伤寒立克次体或立氏立克次体获得的立克次体红细胞致敏物质进行处理。该血清学试剂在室温下至少稳定3个月，在4℃下稳定6个月。它表现出群特异性，无群间交叉反应。在最低稀释度为1:40时，急性和早期恢复期的流行性斑疹伤寒和鼠型斑疹伤寒抗血清显示86%的阳性反应者，而类似的斑点热抗血清有74%的阳性反应者。与间接荧光抗体试验相比，IHA程序的滴度较低，但对大多数配对血清的血清转化检测效果相当。IHA试验显示的滴度明显高于补体结合试验，在识别血清转化方面比补体结合试验或外斐试验更敏感。用已知含有立克次体抗体的啮齿动物血清检测致敏红细胞时未观察到凝集现象。
