Migliorini P, Trovatello G, Cantarella S, Manca F, Bombardieri S, Celada F
J Immunol Methods. 1983 Apr 29;59(2):245-54. doi: 10.1016/0022-1759(83)90037-6.
An enzymatically active probe (beta-galactosidase-anti-beta-galactosidase complex) is used to measure circulating immune complexes (CIC), in a competition assay where probe and CIC are confronted with a 'recognition unit'. The latter is bovine conglutinin in the original description of this method. Here we describe a version utilizing human or bovine C1q. The two techniques are compared for their sensitivity and specificity, on both in vitro formed tetanus toxoid-anti-toxoid complexes and on sera from patients with selected diseases. The results confirm that the two recognition units are sensitive to families of CIC that only partially overlap. The parallel use of conglutinin and C1q yields both quantitative and qualitative information on the nature of CIC in individual sera.
一种具有酶活性的探针（β-半乳糖苷酶-抗β-半乳糖苷酶复合物）用于在竞争分析中测量循环免疫复合物（CIC），在该分析中，探针和CIC与一个“识别单位”相互竞争。在该方法最初的描述中，后者是牛胶固素。在此，我们描述一种使用人或牛C1q的方法。对这两种技术在体外形成的破伤风类毒素-抗毒素复合物以及患有特定疾病患者的血清上的敏感性和特异性进行了比较。结果证实，这两种识别单位对仅部分重叠的CIC家族敏感。同时使用胶固素和C1q可提供关于个体血清中CIC性质的定量和定性信息。
