采用酶免疫测定法检测人血清中的循环免疫复合物。

Measurement of circulating immune complexes in human sera by enzyme immunoassay.

作者信息

Cheung K, O'Kane D, Robinson M F

出版信息

Aust J Exp Biol Med Sci. 1982 Dec;60(6):593-605. doi: 10.1038/icb.1982.62.

DOI:10.1038/icb.1982.62

PMID:6820632

Abstract

Two methods, based on enzyme immunoassay, are described for the detection and quantitation of circulating immune complexes (CIC) in human sera. Aggregated human gamma globulin (AHG) or immune complexes in human sera are bound to complement receptors on Raji cells or to Clq adsorbed on to the plastic surface of microtitre plates. The bound complexes are subsequently detected using peroxidase-conjugated anti-human immunoglobulin antisera. The assays offer a benefit over previously described assays in that they use cheap, commercially available antisera and enable the detection of immune complexes composed of varying immunoglobulin classes.

摘要

本文描述了两种基于酶免疫测定法的方法，用于检测和定量人血清中的循环免疫复合物（CIC）。人血清中的聚集人γ球蛋白（AHG）或免疫复合物与Raji细胞上的补体受体或吸附在微量滴定板塑料表面的Clq结合。随后使用过氧化物酶偶联的抗人免疫球蛋白抗血清检测结合的复合物。这些测定法比先前描述的测定法具有优势，因为它们使用廉价的市售抗血清，并能够检测由不同免疫球蛋白类别组成的免疫复合物。