Siklenka P, Pleva J, Baranová M, Mal'a P, Poliaková A
Vet Med (Praha). 1983 Feb;28(2):105-10.
Differences were studied in detecting residues of inhibitory substances in tissue samples of pigs and calves after emergency slaughter. Samples of liver, heart, kidney and muscle of 30 pigs and 16 calves were examined. Solid samples of organ tissues placed on an agar medium and extracts of the solid samples placed into agar pits were used for detection. Samples were parallelly subjected to the microbiologic diffusion method, using the S. aureus CCM 2022 and B. subtilis CCM 1999 microorganisms. The method of obtaining the extracts from solid samples of the organs was proposed and tested at our own workplace. The presence of inhibitory substances was displayed by the formation of inhibition zones. Results of the positive samples in calves (7 samples) and in pigs (3 samples) point out explicitly to the fact that the extracts of the tissue samples display positively larger inhibition zones in comparison with the solid samples. B. subtilis was demonstrated to be more sensitive than S. aureus, comparing the used microorganisms.
对紧急屠宰后的猪和小牛组织样本中抑制性物质残留的检测差异进行了研究。检查了30头猪和16头小牛的肝脏、心脏、肾脏和肌肉样本。将器官组织的固体样本置于琼脂培养基上，并将固体样本的提取物置于琼脂孔中进行检测。使用金黄色葡萄球菌CCM 2022和枯草芽孢杆菌CCM 1999微生物，对样本平行采用微生物扩散法。提出并在我们自己的工作场所测试了从器官固体样本中获取提取物的方法。抑制性物质的存在通过抑制圈的形成来显示。小牛（7个样本）和猪（3个样本）中阳性样本的结果明确指出，与固体样本相比，组织样本的提取物显示出明显更大的抑制圈。比较所使用的微生物，证明枯草芽孢杆菌比金黄色葡萄球菌更敏感。
