Lippes J, van Oss C J, Bronson P M, Alfonso L A, Dacalos E A, Lucero R
Fertil Steril. 1983 Jun;39(6):824-8. doi: 10.1016/s0015-0282(16)47124-4.
Rabbit antisera elicited by injection of human oviductal fluid (HOF) and rendered specific by absorption with human kidney and human serum revealed the presence of two possibly HOF-specific antigen(s). One antigen was detected by immunodiffusion in 14 of the 14 fluids tested with one of these antisera. HOF-specific antigen was shown to be a beta-globulin by immunoelectrophoresis. A second antigen was seen in two samples. That autoantibodies might exist directed against HOF-specific antigen in the donor's serum was suggested by immunodiffusion experiments in which 4 of the 13 sera tested gave lines of precipitation when reacted against the corresponding oviductal fluid. In one instance, this line was the same as one of the two lines seen in tests using rabbit antiserum.
通过注射人输卵管液(HOF)引发并用人肾和人血清吸收使其具有特异性的兔抗血清显示存在两种可能的HOF特异性抗原。用其中一种抗血清检测的14份液体中有14份通过免疫扩散检测到一种抗原。通过免疫电泳显示HOF特异性抗原是一种β球蛋白。在两个样本中发现了第二种抗原。免疫扩散实验表明供体血清中可能存在针对HOF特异性抗原的自身抗体,在检测的13份血清中有4份与相应的输卵管液反应时产生了沉淀线。在一个实例中,这条线与使用兔抗血清检测时看到的两条线之一相同。