Garnis S, Lala P K
Immunology. 1978 Mar;34(3):487-99.
Small lymphocytes sampled from intraperitoneally growing Ehrlich ascites tumour in CBA/H-T mice as well as host spleen and blood at different days of tumour development were characterized radioautographically on the basis of two surface markers, IgM for B cells and θ antigen for T cells. A direct binding of I-labelled anti-IgM detected natural surface IgM, while an indirect binding following a prior exposure to anti-θ antibody detected θ antigen. Cells remaining unlabelled with the latter procedure were considered to lack both markers (double negative). While the incidence of IgM+ve small lymphocytes within the tumour declined, their absolute numbers increased with tumour growth. Low levels of antiglobulin binding shown by these cells were considered to reflect low levels of maturation, because (1) our previous studies indicated that they were newly formed, and (2) the extent of antiglobulin binding by B lymphocytes in the marrow is known to increase with increasing post-mitotic age. The proportions and the absolute numbers of θ+ve as well as the double negative small lymphocytes increased within the growing tumours. Within the host spleen, the incidence of IgM+ve small lymphocytes remained unchanged but their absolute numbers increased because of splenomegaly. The degree of antiglobulin binding by these cells was comparable to that of the normal splenic population. The incidence of θ+ve cells dropped but their absolute numbers remained unchanged in the spleen during tumour growth. In contrast, the incidence as well as the absolute numbers of double negative cells increased markedly. This cell category increased also in the blood, possibly in transit to the tumour site and other lymphoid organs from the bone marrow, where they were most prevalent. Their bone marrow origin was further suggested by a preponderance of marrow derived small lymphocytes at the tumour site as well as in the host spleens found in our earlier studies. Double negative population in the spleen showed a paucity of C′3 and Fc receptors on the cell surface and included cells capable of producing B lymphoid colonies .
从CBA/H-T小鼠腹腔内生长的艾氏腹水瘤以及肿瘤发展不同阶段的宿主脾脏和血液中采集的小淋巴细胞，根据两种表面标志物进行放射自显影表征，B细胞的IgM和T细胞的θ抗原。I标记的抗IgM的直接结合检测到天然表面IgM，而预先暴露于抗θ抗体后的间接结合检测到θ抗原。后一种方法未标记的细胞被认为缺乏这两种标志物（双阴性）。虽然肿瘤内IgM阳性小淋巴细胞的发生率下降，但其绝对数量随肿瘤生长而增加。这些细胞显示的低水平抗球蛋白结合被认为反映了低水平的成熟，因为（1）我们之前的研究表明它们是新形成的，（2）已知骨髓中B淋巴细胞的抗球蛋白结合程度会随着有丝分裂后年龄的增加而增加。在生长的肿瘤内，θ阳性以及双阴性小淋巴细胞的比例和绝对数量增加。在宿主脾脏中，IgM阳性小淋巴细胞的发生率保持不变，但其绝对数量因脾肿大而增加。这些细胞的抗球蛋白结合程度与正常脾脏群体相当。在肿瘤生长过程中，脾脏中θ阳性细胞的发生率下降，但其绝对数量保持不变。相比之下，双阴性细胞的发生率和绝对数量均显著增加。这类细胞在血液中也增加，可能是从骨髓转运到肿瘤部位和其他淋巴器官，骨髓是它们最常见的地方。我们早期研究发现肿瘤部位以及宿主脾脏中骨髓来源的小淋巴细胞占优势，进一步表明它们起源于骨髓。脾脏中的双阴性群体在细胞表面显示出缺乏C′3和Fc受体，并且包括能够产生B淋巴集落的细胞。
