Mulé J J, Hellström I, Hellström K E
Am J Pathol. 1982 May;107(2):142-9.
Using a cell separation technique to isolate mononuclear leukocytes from solid tumors in conjunction with combined radioautography and two-color immunofluorescence, we have characterized the surface markers of 3H-labeled, immune, long-lived, small lymphocytes (LLSLs) that selectively localize into chemically induced sarcomas. When small tumor pieces were implanted into immune mice whose LLSLs had been radiolabeled, the LLSLs that selectively localized into those tumors to which the hosts had been immunized were predominantly Thy-1+, Lyt-1+2-. Localization into the appropriate tumor was also detected upon adoptive transfer of immune LLSLs to tumor-bearing mice, especially when these mice had, before transfer, received 400 rads of whole-body irradiation. These 3H-labeled LLSLs were also found to be predominantly Thy-1+, Lyt-1+2-.
运用细胞分离技术从实体瘤中分离单核白细胞，并结合放射自显影和双色免疫荧光技术，我们已对选择性定位于化学诱导肉瘤中的3H标记的免疫长寿小淋巴细胞（LLSLs）的表面标志物进行了表征。当将小肿瘤块植入其LLSLs已被放射性标记的免疫小鼠体内时，选择性定位于宿主已免疫的那些肿瘤中的LLSLs主要为Thy-1 +、Lyt-1 + 2 -。将免疫LLSLs过继转移至荷瘤小鼠时，也检测到其定位于合适的肿瘤中，尤其是当这些小鼠在转移前接受了400拉德的全身照射时。这些3H标记的LLSLs也主要为Thy-1 +、Lyt-1 + 2 -。
