Berman J D, Johnson W D
Infect Immun. 1978 Mar;19(3):898-902. doi: 10.1128/iai.19.3.898-902.1978.
Monocytes of healthy, full-term newborns were isolated from cord blood, and functional and biochemical activities were quantitated. The yield of monocytes per milliliter of cord blood was 60% greater than that from the peripheral blood of healthy adults. Placental monocytes were initially less well spread than cells from adults, but no other morphological differences were noted. During 4 days of in vitro cultivation, placental monocytes secreted lysozyme at a constant rate, lost peroxidase activity, and increased 5'-nucleotidase activity 15- to 25-fold. Similar findings were obtained with monocytes from adults. Placental monocytes also displayed Fc and complement receptor activity. Ingestion and intracellular multiplication of Toxoplasma gondii were identical in normal placental and adult monocytes. Furthermore, toxoplasma multiplication was significantly inhibited by cells from both sources when the monocytes were preincubated with supernatants prepared from sensitized lymphocytes and toxoplasma antigen. Monocytes from newborns were competent cells in terms of the specific functions and activities we examined.
从健康足月新生儿的脐带血中分离出单核细胞，并对其功能和生化活性进行定量分析。每毫升脐带血中单核细胞的产量比健康成年人外周血中的产量高60%。胎盘单核细胞最初比成年人的细胞铺展性差，但未观察到其他形态学差异。在体外培养的4天中，胎盘单核细胞以恒定速率分泌溶菌酶，失去过氧化物酶活性，5'-核苷酸酶活性增加15至25倍。成年人的单核细胞也有类似的发现。胎盘单核细胞还表现出Fc和补体受体活性。正常胎盘单核细胞和成年单核细胞对弓形虫的摄取和细胞内增殖情况相同。此外，当单核细胞与由致敏淋巴细胞和弓形虫抗原制备的上清液预孵育时，来自这两种来源的细胞均能显著抑制弓形虫的增殖。就我们所检测的特定功能和活性而言，新生儿的单核细胞是有功能的细胞。
