Shimizu M, Wajima O, Miura M, Katayama I
Cancer. 1983 Aug 1;52(3):486-92. doi: 10.1002/1097-0142(19830801)52:3<486::aid-cncr2820520318>3.0.co;2-t.
A PAP immunoperoxidase method for demonstrating estrogen receptors in breast carcinomas is introduced. Whereas other methods pretreat the specimen with exogenous estrogen in high (unphysiologic) doses, this method demonstrates endogenous estrogen without such pretreatment. Experiments showed endogenous estrogen remaining in the formalin-fixed, paraffin-embedded breast tumors in sufficient amounts to be detected by the PAP method. The method can discriminate hormone responsive cells from hormone secreting cells because most responsive cells demonstrate both the cytoplasmic and nuclear stain and most secreting cells only the cytoplasmic stain. The method was applied to 277 human breast carcinomas, of which 197 (71.1%) were positive and 80 (28.9%) negative. These figures were comparable to those obtained by the biochemical methods. This PAP method may serve as an alternative to the biochemical assays of estrogen receptors.
本文介绍了一种用于显示乳腺癌中雌激素受体的PAP免疫过氧化物酶法。其他方法是用高剂量（非生理剂量）的外源性雌激素对标本进行预处理，而该方法无需这种预处理就能显示内源性雌激素。实验表明，在福尔马林固定、石蜡包埋的乳腺肿瘤中残留有足够量的内源性雌激素，可用PAP法检测到。该方法能够区分激素反应性细胞和激素分泌性细胞，因为大多数反应性细胞在细胞质和细胞核中均有染色，而大多数分泌性细胞仅在细胞质中有染色。该方法应用于277例人类乳腺癌，其中197例（71.1%）为阳性，80例（28.9%）为阴性。这些数据与通过生化方法获得的数据相当。这种PAP法可作为雌激素受体生化检测的替代方法。
