Katayama I, Shimizu M, Miura M, Maruyama M, Kobayashi M, Iino Y, Izuo M, Wakatsuki S
Virchows Arch A Pathol Anat Histopathol. 1984;402(4):353-9. doi: 10.1007/BF00734633.
In a study of 277 patients with breast carcinomas, the PAP immunoperoxidase method for demonstrating endogenous estrogen was correlated with the sucrose density gradient (SDG) assay and with histologic and clinical features. The results from the PAP method and SDG assay agreed in 59 of 84 patients (82.1%) on whom both methods were performed. Histologically, the PAP method was positive in 7 of 7 patients with non-invasive carcinomas, in 164 of 233 patients (70.4%) with common invasive ductal carcinoma, and in 21 of 22 of those with special histological types of of invasive carcinomas not including Paget's disease, medullary or apocrine carcinoma, where only 5 of 14 were positive. Clinically, 15 of 18 patients with positive endogenous estrogen showed a response to endocrine therapy as opposed to 1 of 9 patients with a negative endogenous estrogen. The mean survival was 31.2 and 15.6 months, respectively for patients with positive and negative endogenous estrogen. Remission for longer than 2 years was seen more often in patients with positive endogenous estrogen. These results suggest a clinical utility of the present PAP method which, therefore, deserves a further trial as an alternative to histochemical methods aiming at the estrogen receptors.
在一项针对277例乳腺癌患者的研究中，用于检测内源性雌激素的PAP免疫过氧化物酶法与蔗糖密度梯度（SDG）分析以及组织学和临床特征相关。在84例同时采用这两种方法检测的患者中，PAP法和SDG分析结果在59例患者（82.1%）中一致。组织学上，PAP法在7例非浸润性癌患者中全部呈阳性，在233例常见浸润性导管癌患者中有164例（70.4%）呈阳性，在22例不包括派杰病、髓样癌或大汗腺癌的特殊组织学类型浸润性癌患者中有21例呈阳性，而在14例此类患者中只有5例呈阳性。临床上，18例内源性雌激素呈阳性的患者中有15例对内分泌治疗有反应，而内源性雌激素呈阴性的9例患者中只有1例有反应。内源性雌激素呈阳性和阴性的患者平均生存期分别为31.2个月和15.6个月。内源性雌激素呈阳性的患者更常出现超过2年的缓解期。这些结果表明当前PAP法具有临床实用性，因此，作为一种替代旨在检测雌激素受体的组织化学方法，值得进一步试验。
