Franko M C, Gibbs C J, Lee P W, Gajdusek D C
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4149-53. doi: 10.1073/pnas.80.13.4149.
Six hybridoma cell line producing monoclonal antibodies to Hantaan virus were established by fusion of NS-1 mouse myeloma cells with spleen cells of mice immunized with Hantaan virus strain 76-118. The specificity of these monoclonal antibodies was established by immunoblotting analysis and immunofluorescence. Five of the clones reacted with antigens on the cell surface and in the cytoplasm, and one clone reacted with a determinant expressed only in the cytoplasm of the infected cells. Two of the clones produced antibodies that reacted with a Mr 50,000 polypeptide in virus-infected cellular extracts and purified virus preparations. The monoclonal antibodies were used to examine the antigenic relationship among Hantaan virus strains and between Hantaan virus and Prospect Hill virus and the virus of nephropathia epidemica. Three antibodies were capable of distinguishing between the Lee strain and the 760-118 strain of Hantaan virus and three additional antibodies reacted with determinants shared by both virus strains. None of the six reacted with Prospect Hill virus or the virus of nephropathia epidemica.
通过将NS-1小鼠骨髓瘤细胞与用汉滩病毒76-118株免疫的小鼠脾细胞融合，建立了6株产生抗汉滩病毒单克隆抗体的杂交瘤细胞系。这些单克隆抗体的特异性通过免疫印迹分析和免疫荧光确定。其中5个克隆与细胞表面和细胞质中的抗原发生反应，1个克隆与仅在感染细胞细胞质中表达的一个决定簇发生反应。2个克隆产生的抗体与病毒感染细胞提取物和纯化病毒制剂中的一种分子量为50000的多肽发生反应。这些单克隆抗体用于检测汉滩病毒株之间以及汉滩病毒与普洛威顿斯山病毒和流行性肾病病毒之间的抗原关系。3种抗体能够区分汉滩病毒的Lee株和76-118株，另外3种抗体与两种病毒株共有的决定簇发生反应。这6种抗体均不与普洛威顿斯山病毒或流行性肾病病毒发生反应。
