Zöller L, Scholz J, Stohwasser R, Giebel L B, Sethi K K, Bautz E K, Darai G
Institut für Medizinische Virologie, Universität Heidelberg, Federal Republic of Germany.
J Med Virol. 1989 Mar;27(3):231-7. doi: 10.1002/jmv.1890270309.
Sera from patients with nephropathia epidemica (NE) or Korean hemorrhagic fever (KHF) were tested for specific antibody response to antigens of Hällnäs virus and Hantaan virus strain 76-118. A Vero E6 derived cell line persistently infected with Hällnäs virus strain B1, and Vero E6 cells freshly infected with Hantaan virus type strain 76-118 were used as antigens in the immunofluorescence assay (IFA) and the immunoblot. Blots were prepared from whole cell lysates. The convalescent-phase sera of NE patients tested in this study regularly revealed a marked reaction with a 52 kilodalton (Kd) protein of Hällnäs virus and a 50 Kd protein of Hantaan virus. A convalescent serum from a patient with Korean hemorrhagic fever and a rat antiserum against Hantaan virus could recognize the 50 Kd band of Hantaan virus but showed no apparent reactivity with the 52 Kd component of Hällnäs virus in the standard dilutions. Some sera could additionally identify minor bands in the 55 Kd and/or 67 Kd region of the blots. A one-way cross reactivity between Hantaan and Hällnäs viruses was also evident from the results of the immunofluorescence assays in that NE convalescent sera reacted with both viruses, whereas KHF convalescent or anti-Hantaan sera gave strongly positive results with Hantaan virus but only faint reaction with Hällnäs virus.
对肾病综合征出血热（NE）或朝鲜出血热（KHF）患者的血清进行检测，以观察其对哈尔纳斯病毒和汉坦病毒76 - 118株抗原的特异性抗体反应。将持续感染哈尔纳斯病毒B1株的Vero E6衍生细胞系以及刚感染汉坦病毒原型株76 - 118的Vero E6细胞用作免疫荧光测定（IFA）和免疫印迹中的抗原。印迹由全细胞裂解物制备。本研究中检测的NE患者恢复期血清经常显示与哈尔纳斯病毒的一种52千道尔顿（Kd）蛋白和汉坦病毒的一种50 Kd蛋白有明显反应。一名朝鲜出血热患者的恢复期血清和一种抗汉坦病毒的大鼠抗血清在标准稀释度下可识别汉坦病毒的50 Kd条带，但与哈尔纳斯病毒的52 Kd成分无明显反应。一些血清还可在印迹的55 Kd和/或67 Kd区域识别出较小的条带。免疫荧光测定结果也表明汉坦病毒和哈尔纳斯病毒之间存在单向交叉反应，即NE恢复期血清与两种病毒都有反应，而KHF恢复期血清或抗汉坦血清对汉坦病毒呈强阳性反应，但对哈尔纳斯病毒只有微弱反应。
