Ruo S L, Sanchez A, Elliott L H, Brammer L S, McCormick J B, Fisher-Hoch S P
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia.
Arch Virol. 1991;119(1-2):1-11. doi: 10.1007/BF01314318.
Thirty hybrid cell lines that produce monoclonal antibodies to three strains of hantaviruses have been generated and characterized. One clone specific to Hantaan 76-118 strain, four clones specific to Rattus strains and one clone specific to Puumala virus have been identified. Most of the monoclones produced antibodies specific to nucleoproteins. Only two monoclones were found to produce glycoprotein specific, neutralizing antibodies. The immunofluorescent (IFA) staining patterns of the monoclonal antibodies show consistent correlation with viral protein specificities as described for other hemorrhagic fever viruses. Cross-reactivity studies with hantaviruses tested demonstrate conserved antigenic sites on nucleoproteins among these hantaviruses tested. Puumala specific monoclones, produced for the first time, reveal both conserved and strain specific sites on the viral nucleoproteins of the Scandinavian virus.
已经产生并鉴定了30个针对三种汉坦病毒株产生单克隆抗体的杂交细胞系。已鉴定出一个针对汉滩76 - 118株的克隆、四个针对褐家鼠株的克隆和一个针对普马拉病毒的克隆。大多数单克隆产生针对核蛋白的特异性抗体。仅发现两个单克隆产生糖蛋白特异性中和抗体。单克隆抗体的免疫荧光（IFA）染色模式与其他出血热病毒所描述的病毒蛋白特异性显示出一致的相关性。对所测试的汉坦病毒进行的交叉反应性研究表明，在所测试的这些汉坦病毒中，核蛋白上存在保守的抗原位点。首次产生的普马拉特异性单克隆揭示了斯堪的纳维亚病毒的病毒核蛋白上既有保守位点也有株特异性位点。
