Ha I S, Choi Y, Park Y S, Cheong H I, Koo J W, Kim I S, Kim E C, Kim S, Lee J S
Department of Pediatrics, Seoul National University College of Medicine, Republic of Korea.
Clin Diagn Lab Immunol. 1995 Jul;2(4):439-42. doi: 10.1128/cdli.2.4.439-442.1995.
Forty-five hybridoma cell lines producing monoclonal antibodies against Hantaan virus, the etiologic agent of hemorrhagic fever with renal syndrome, were generated by fusion of P3-X63-Ag8.V653 myeloma cells with spleen cells of mice immunized with inactivated Hantaan virus vaccine. Among these, 38 antibodies were identified as binding to the 48-kDa nucleocapsid protein by immunoblot assay or radioimmunoprecipitation. Twenty-six of them were of the immunoglobulin G1 (IgG1), nine were of the IgG2a, and three were of the IgA isotype. According to cross-reactivities with other serotypes of the genus Hantavirus, the antibodies were classified into three groups: 6 antibodies specific to the Hantaan serotype (group I), 20 antibodies cross-reacting with Hantaan and Seoul serotypes (SR-11, Tchoupitoulas, and R22) (group II), and 12 antibodies cross-reacting with Hantaan, Seoul, and Prospect Hill serotypes (group III). None of the antibodies cross-reacted with the Puumala serotype. With a panel of antibodies of different cross-reactivities, serotypes of Hantavirus could be differentiated. Thirty-eight monoclonal antibodies against Hantaan virus nucleocapsid protein which have different cross-reactivities between serotypes were developed. These results confirmed the presence of multiple serotype-specific epitopes on the nucleocapsid protein of Hantaan virus, which can be utilized in differentiation of serotypes.
用灭活的汉坦病毒疫苗免疫小鼠，取其脾细胞与P3-X63-Ag8.V653骨髓瘤细胞融合，产生了45个针对肾综合征出血热病原体汉坦病毒的杂交瘤细胞系。其中，通过免疫印迹分析或放射免疫沉淀法鉴定出38种抗体与48 kDa核衣壳蛋白结合。其中26种为免疫球蛋白G1（IgG1），9种为IgG2a，3种为IgA同种型。根据与汉坦病毒属其他血清型的交叉反应性，这些抗体分为三组：6种对汉坦血清型特异的抗体（I组），20种与汉坦和汉城血清型（SR-11、Tchoupitoulas和R22）交叉反应的抗体（II组），以及12种与汉坦、汉城和普马拉血清型交叉反应的抗体（III组）。没有一种抗体与普马拉血清型交叉反应。利用一组具有不同交叉反应性的抗体，可以区分汉坦病毒的血清型。开发了38种针对汉坦病毒核衣壳蛋白的单克隆抗体，它们在血清型之间具有不同的交叉反应性。这些结果证实了汉坦病毒核衣壳蛋白上存在多个血清型特异性表位，可用于血清型的区分。
