Kessler R E, Thivierge B H
Infect Immun. 1983 Aug;41(2):549-55. doi: 10.1128/iai.41.2.549-555.1983.
The influence of D-alanine and carbohydrate substitution of lipoteichoic acids (LTAs) on the binding of antibody directed to the polyglycerol phosphate (PGP) portion was found to be at least partially dependent upon the mode of presentation of the antigen. There were greater differences in binding of anti-PGP immunoglobulins to substituted and unsubstituted LTAs in solution (micellar presentation) than when the same LTAs were adsorbed to the erythrocyte surface, which suggests that there is greater hindrance of access to the PGP chain, possibly as a result of closer packing, in a vesicle or micelle than when LTA is bound to a membrane surface. Although the difference in binding of anti-PGP immunoglobulins was nearly 20-fold between unsubstituted and highly substituted LTAs, rocket heights by rocket immunoelectrophoresis were only 4-fold different for the most highly substituted LTA and unsubstituted LTA. However, unsubstituted LTA clearly bound more immunoglobulin molecules than was reflected in the rocket height alone, since the resulting immunoprecipitates were much more prominent or intense, both before and after staining, than those of highly substituted LTAs. Differences between lightly and moderately substituted LTAs were less than twofold, indicating that under most circumstances estimates of LTA concentrations in samples where the composition is unknown will be within approximately twofold of the estimate.
研究发现，D-丙氨酸和脂磷壁酸（LTA）的碳水化合物取代对针对聚甘油磷酸（PGP）部分的抗体结合的影响至少部分取决于抗原的呈现方式。与相同的LTA吸附到红细胞表面时相比，抗PGP免疫球蛋白在溶液中（胶束呈现）与取代和未取代的LTA的结合差异更大，这表明与LTA结合到膜表面时相比，在囊泡或胶束中，PGP链的可及性受到更大阻碍，这可能是由于堆积更紧密所致。尽管未取代和高度取代的LTA之间抗PGP免疫球蛋白的结合差异近20倍，但对于高度取代的LTA和未取代的LTA，火箭免疫电泳的火箭高度仅相差4倍。然而，未取代的LTA明显结合了更多的免疫球蛋白分子，这不仅仅反映在火箭高度上，因为所得免疫沉淀物在染色前后都比高度取代的LTA的沉淀物更明显或更强烈。轻度和中度取代的LTA之间的差异小于两倍，这表明在大多数情况下，对于成分未知的样品中LTA浓度的估计将在估计值的约两倍范围内。
