枯草芽孢杆菌对趋化性甲基转移酶II的透化作用。
Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II.
作者信息
Burgess-Cassler A, Ordal G W
出版信息
J Bacteriol. 1983 Aug;155(2):907-9. doi: 10.1128/jb.155.2.907-909.1983.
Abstract
Treatment of Bacillus subtilis with 0.4% (vol/vol) toluene renders cells permeable not only to small molecules but also apparently to proteins as large as 30,000 daltons. Methyl-accepting chemotaxis proteins and two smaller polypeptides were methylated when B. subtilis methyltransferase II was added to permeabilized cells.
摘要
用0.4%（体积/体积）甲苯处理枯草芽孢杆菌，不仅会使细胞对小分子具有通透性，而且显然对高达30,000道尔顿的蛋白质也具有通透性。当将枯草芽孢杆菌甲基转移酶II添加到通透细胞中时，甲基接受趋化蛋白和两种较小的多肽会发生甲基化。
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