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枯草芽孢杆菌对趋化性甲基转移酶II的透化作用。

Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II.

作者信息

Burgess-Cassler A, Ordal G W

出版信息

J Bacteriol. 1983 Aug;155(2):907-9. doi: 10.1128/jb.155.2.907-909.1983.

DOI:10.1128/jb.155.2.907-909.1983
PMID:6409887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217768/
Abstract

Treatment of Bacillus subtilis with 0.4% (vol/vol) toluene renders cells permeable not only to small molecules but also apparently to proteins as large as 30,000 daltons. Methyl-accepting chemotaxis proteins and two smaller polypeptides were methylated when B. subtilis methyltransferase II was added to permeabilized cells.

摘要

用0.4%（体积/体积）甲苯处理枯草芽孢杆菌，不仅会使细胞对小分子具有通透性，而且显然对高达30,000道尔顿的蛋白质也具有通透性。当将枯草芽孢杆菌甲基转移酶II添加到通透细胞中时，甲基接受趋化蛋白和两种较小的多肽会发生甲基化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/ab25898cb36b/jbacter00243-0473-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/014e2c09c8c2/jbacter00243-0472-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/a6c50e89999a/jbacter00243-0472-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/ab25898cb36b/jbacter00243-0473-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/014e2c09c8c2/jbacter00243-0472-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/a6c50e89999a/jbacter00243-0472-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9dee/217768/ab25898cb36b/jbacter00243-0473-a.jpg

相似文献

1
Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II.枯草芽孢杆菌对趋化性甲基转移酶II的透化作用。
J Bacteriol. 1983 Aug;155(2):907-9. doi: 10.1128/jb.155.2.907-909.1983.
2
Functional homology of Bacillus subtilis methyltransferase II and Escherichia coli cheR protein.枯草芽孢杆菌甲基转移酶II与大肠杆菌cheR蛋白的功能同源性
J Biol Chem. 1982 Nov 10;257(21):12835-8.
3
Purification and characterization of Bacillus subtilis methyl-accepting chemotaxis protein methyltransferase II.
J Biol Chem. 1982 Jul 25;257(14):8412-7.
4
In vitro methylation and demethylation of methyl-accepting chemotaxis proteins in Bacillus subtilis.枯草芽孢杆菌中甲基受体趋化蛋白的体外甲基化与去甲基化
Biochemistry. 1984 Jun 5;23(12):2600-6. doi: 10.1021/bi00307a010.
5
Methyl group turnover on methyl-accepting chemotaxis proteins during chemotaxis by Bacillus subtilis.枯草芽孢杆菌趋化作用期间甲基接受趋化蛋白上的甲基基团周转
J Biol Chem. 1990 Feb 5;265(4):1928-32.
6
Methyl transfer in chemotaxis toward sugars by Bacillus subtilis.枯草芽孢杆菌对糖类趋化作用中的甲基转移
J Bacteriol. 1990 Feb;172(2):1148-50. doi: 10.1128/jb.172.2.1148-1150.1990.
7
Evidence for methyl group transfer between the methyl-accepting chemotaxis proteins in Bacillus subtilis.枯草芽孢杆菌中甲基接受趋化蛋白之间甲基基团转移的证据。
J Bacteriol. 1988 Jan;170(1):223-7. doi: 10.1128/jb.170.1.223-227.1988.
8
Methyl esterification of glutamic acid residues of methyl-accepting chemotaxis proteins in Bacillus subtilis.枯草芽孢杆菌中甲基接受趋化蛋白谷氨酸残基的甲基酯化作用。
Biochem J. 1983 Sep 1;213(3):759-63. doi: 10.1042/bj2130759.
9
Novel methyl transfer during chemotaxis in Bacillus subtilis.枯草芽孢杆菌趋化作用过程中的新型甲基转移
Biochemistry. 1989 Jun 27;28(13):5585-9. doi: 10.1021/bi00439a037.
10
Rapid attractant-induced changes in methylation of methyl-accepting chemotaxis proteins in Bacillus subtilis.快速引诱剂诱导枯草芽孢杆菌中甲基接受趋化蛋白甲基化的变化。
Biochemistry. 1988 Nov 1;27(22):8453-7. doi: 10.1021/bi00422a024.

本文引用的文献

1
Purification and characterization of Bacillus subtilis methyl-accepting chemotaxis protein methyltransferase II.
J Biol Chem. 1982 Jul 25;257(14):8412-7.
2
Chemotaxis in Bacillus subtilis: effects of attractants on the level of methylation of methyl-accepting chemotaxis proteins and the role of demethylation in the adaptation process.枯草芽孢杆菌中的趋化作用：引诱剂对甲基化趋化蛋白甲基化水平的影响以及去甲基化在适应过程中的作用。
Biochemistry. 1982 Mar 2;21(5):915-20. doi: 10.1021/bi00534a016.
3
In vivo and in vitro chemotactic methylation in Bacillus subtilis.枯草芽孢杆菌体内和体外的趋化甲基化
J Bacteriol. 1981 Feb;145(2):958-65. doi: 10.1128/jb.145.2.958-965.1981.
4
Methylation of chemotaxis-specific proteins in Escherichia coli cells permeable to S-adenosylmethionine.对可渗透S-腺苷甲硫氨酸的大肠杆菌细胞中趋化特异性蛋白的甲基化作用。
Biochemistry. 1980 Sep 30;19(20):4627-32. doi: 10.1021/bi00561a014.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Effects of toluene on Escherichia coli.甲苯对大肠杆菌的影响。
J Bacteriol. 1965 Nov;90(5):1420-5. doi: 10.1128/jb.90.5.1420-1425.1965.
7
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.通过荧光自显影对聚丙烯酰胺凝胶中的³H和¹⁴C进行定量胶片检测。
Eur J Biochem. 1975 Aug 15;56(2):335-41. doi: 10.1111/j.1432-1033.1975.tb02238.x.
8
Chemotaxis away from uncouplers of oxidative phosphorylation in Bacillus subtilis.枯草芽孢杆菌中远离氧化磷酸化解偶联剂的趋化作用。
Science. 1975 Sep 5;189(4205):802-5. doi: 10.1126/science.808854.
9
The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of Escherichia coli.甲苯对大肠杆菌外膜和细胞质膜结构及通透性的影响。
Biochim Biophys Acta. 1978 Jan 4;506(1):64-80. doi: 10.1016/0005-2736(78)90435-2.
10
Permeabilization of cells for studies on the biochemistry of bacterial chemotaxis.用于细菌趋化性生物化学研究的细胞透化处理。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3693-7. doi: 10.1073/pnas.76.8.3693.