Skinner J E, Beder S D, Entman M L
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4513-7. doi: 10.1073/pnas.80.14.4513.
The present study uses a technique that enables the collection of multiple freeze-biopsy samples from the myocardium of the conscious pig (i.e., through a thoracic window). This technique enables sequential analysis of the metabolic state of the myocardium during different behavioral conditions. The results demonstrate that with daily adaptations to an unfamiliar environment (i.e., stress reduction), the phosphorylase activation ratio (phosphorylase a/total phosphorylase) in the quiescent pig declines steadily from approximately 80% to 30% (r = -0.91, P less than 0.01). This decline occurs with both the mean resting heart rate and left ventricular blood pressure remaining constant. The decline is seen within individual subjects during the whole adaptation sequence as well as between subjects whose samples were taken either early or late in the adaptation series. The dissociation of hemodynamic functional and metabolic activation in the unadapted, psychologically stressed pig may be associated with the occurrence of increased vulnerability of the ischemic heart to ventricular fibrillation, a phenomenon previously observed under the same behavioral conditions.
本研究采用了一种技术，能够从清醒猪的心肌中采集多个冷冻活检样本（即通过胸腔窗口）。该技术能够在不同行为条件下对心肌的代谢状态进行连续分析。结果表明，随着每日适应不熟悉的环境（即应激减轻），安静状态下猪的磷酸化酶激活率（磷酸化酶a/总磷酸化酶）从约80%稳步下降至30%（r = -0.91，P小于0.01）。这种下降发生在平均静息心率和左心室血压保持恒定的情况下。在整个适应过程中，个体受试者以及在适应系列早期或晚期采集样本的受试者之间均出现了这种下降。在未适应、心理应激的猪中，血流动力学功能与代谢激活的分离可能与缺血性心脏对室颤易感性增加的发生有关，这是先前在相同行为条件下观察到的一种现象。
