[含葡萄球菌质粒DNA的粗裂解物对枯草芽孢杆菌的转化]
[Transformation of Bacillus subtilis by crude lysates containing staphylococcal plasmid DNA].
作者信息
Ivanov N A, Danilova E G, Medvedkova N A, Pekhov A P
出版信息
Biull Eksp Biol Med. 1983 Aug;96(8):78-80.
PMID:6411147
Abstract
Crude lysates from staphylococcal strains, containing DNA, were capable of transforming Bacillus subtilis at a rate of 1.68 X 10(-10) - 20.6 X 10(-10) depending on the marker according to which the transformers were selected. In a new host, plasmids showed the same behavior pattern as in the staphylococcus but their spontaneous loss was in all the cases recorded significantly more often.
摘要
含有DNA的葡萄球菌菌株的粗裂解物能够以1.68×10⁻¹⁰ - 20.6×10⁻¹⁰的转化率转化枯草芽孢杆菌，转化率取决于用于选择转化体的标记。在新宿主中，质粒表现出与在葡萄球菌中相同的行为模式，但在所有记录的情况下，它们的自发丢失频率明显更高。
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