Feitelson J S, Lederberg J
J Gen Microbiol. 1980 Feb;116(2):545-7. doi: 10.1099/00221287-116-2-545.
Plasmids can be transferred from Staphylococcus aureus to Bacillus subtilis by crude lysates prepared with penicillin or lysostaphin. These lysates mediate drug-resistance plasmid transformation in competent B. subtilis at an efficiency paralleling that of purified DNA.
用青霉素或溶葡萄球菌素制备的粗裂解物可将质粒从金黄色葡萄球菌转移至枯草芽孢杆菌。这些裂解物介导耐药性质粒在感受态枯草芽孢杆菌中的转化,其效率与纯化DNA相当。