Bridges R B, Hsieh L, Haack D G
Infect Immun. 1980 Sep;29(3):1096-101. doi: 10.1128/iai.29.3.1096-1101.1980.
The in vitro effects of the water-soluble fraction of whole cigarette smoke (WSF) and two alpha, beta-unsaturated aldehydes of cigarette smoke (acrolein and crotonaldehyde) on polymorphonuclear leukocyte (PMNL) adherence were determined with nylon fiber columns. Each of these cigarette smoke constituents caused a dose-dependent inhibition of PMNL adherence. However, at least fivefold higher concentrations of these agents were necessary to inhibit adherence as compared with those necessary to achieve the same level of inhibition of PMNL chemotaxis. Furthermore, inhibition of adherence by WSF could be differentiated from its effects on chemotaxis in that reduced glutathione completely protected chemotaxis from the effects of WSF but only afforded partial protection to PMNL adherence. These data suggest that the inhibitory effects of WSF, acrolein, and crotonaldehyde on PMNL chemotaxis are not due to their inhibition of adherence. Finally, although PMNL adherence is considered to be an integral part of the chemotactic mechanism, differentiation between these two PMNL functions may be possible, since some inhibitors of chemotaxis do not have corresponding inhibitory effects on adherence.
采用尼龙纤维柱测定了全香烟烟雾的水溶性成分（WSF）以及香烟烟雾中的两种α，β-不饱和醛（丙烯醛和巴豆醛）对多形核白细胞（PMNL）黏附的体外作用。这些香烟烟雾成分中的每一种都会引起PMNL黏附的剂量依赖性抑制。然而，与实现相同水平的PMNL趋化性抑制所需的浓度相比，这些物质抑制黏附所需的浓度至少高五倍。此外，WSF对黏附的抑制作用可与其对趋化性的影响区分开来，因为还原型谷胱甘肽能完全保护趋化性免受WSF的影响，但仅对PMNL黏附提供部分保护。这些数据表明，WSF、丙烯醛和巴豆醛对PMNL趋化性的抑制作用并非由于它们对黏附的抑制。最后，虽然PMNL黏附被认为是趋化机制的一个组成部分，但这两种PMNL功能之间可能存在差异，因为一些趋化性抑制剂对黏附没有相应的抑制作用。
