Kauffman H F, Beaumont F, Meurs H, van der Heide S, de Vries K
J Allergy Clin Immunol. 1983 Sep;72(3):255-61. doi: 10.1016/0091-6749(83)90029-5.
IgG antibody titers against Aspergillus fumigatus from different sera were measured by means of a standardized ELISA and compared with precipitates measured by double diffusion (D.D.). There was a significant correlation between the number of precipitates and the ELISA IgG titers in the 758 sera examined. However, in several individual sera within this group, large deviations between these two immunologic parameters were found. Further analysis indicated that ELISA detects antibodies against nonprecipitating antigenic components in addition to the antibodies detected by D.D. Furthermore, not all precipitating antigenic components appear to play a role in the detection of antibodies against A. fumigatus by ELISA. Patients with aspergillosis largely show increased titers of IgG antibody by ELISA even when the results of D.D. are negative, except those of patients with Aspergillus-provoked asthma which fall within a normal range.
通过标准化酶联免疫吸附测定法（ELISA）测量不同血清中针对烟曲霉的IgG抗体滴度，并与通过双向扩散法（D.D.）测量的沉淀物进行比较。在所检测的758份血清中，沉淀物数量与ELISA IgG滴度之间存在显著相关性。然而，在该组中的几个个体血清中，发现这两个免疫参数之间存在较大偏差。进一步分析表明，ELISA除了检测到D.D.所检测的抗体外，还能检测针对非沉淀性抗原成分的抗体。此外，并非所有沉淀性抗原成分似乎都在ELISA检测针对烟曲霉的抗体中发挥作用。曲霉病患者即使D.D.结果为阴性，ELISA检测的IgG抗体滴度大多也会升高，但曲霉诱发哮喘患者的结果在正常范围内除外。
