Brouwer J
Stichting Samenwerking Delftse Ziekenhuizen (SSDZ), Delft, The Netherlands.
Int Arch Allergy Appl Immunol. 1988;85(2):244-9. doi: 10.1159/000234510.
The performance of ELISA to detect IgG and IgM antibodies to Aspergillus fumigatus has been evaluated in strongly precipitin-positive, weakly precipitin-positive and precipitin-negative patient sera, with immunoblot analysis as the confirmatory test. All strongly precipitin-positive sera contained increased IgG titers and showed clearly positive immunoblot patterns. Most of the weakly precipitin-positive sera contained ELISA titers within the normal range established with sera of healthy blood donors and showed normal immunoblot patterns. Increased titers of IgG and/or IgM were measured in one-sixth of the precipitin-negative patient sera. Immunoblot analysis confirmed the presence of antibodies to A. fumigatus in 55% of the precipitin-negative sera with increased antibody titers. ELISAs for A. fumigatus-specific IgG and IgM are sensitive tests for screening of patient sera. However, positive results with ELISA should be confirmed by means of immunoblot analysis.
以免疫印迹分析作为确证试验，在强沉淀素阳性、弱沉淀素阳性和沉淀素阴性患者血清中评估了检测烟曲霉IgG和IgM抗体的ELISA性能。所有强沉淀素阳性血清的IgG滴度均升高，且免疫印迹模式呈明显阳性。大多数弱沉淀素阳性血清的ELISA滴度在以健康献血者血清确定的正常范围内，且免疫印迹模式正常。在六分之一的沉淀素阴性患者血清中检测到IgG和/或IgM滴度升高。免疫印迹分析证实，在55%抗体滴度升高的沉淀素阴性血清中存在抗烟曲霉抗体。检测烟曲霉特异性IgG和IgM的ELISA是筛查患者血清的敏感试验。然而，ELISA的阳性结果应以免疫印迹分析进行确证。
