用于检测烟曲霉抗体的快速酶联免疫吸附测定（ELISA）。
Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergillus fumigatus antibodies.
作者信息
Richardson M D, Stubbins J M, Warnock D W
出版信息
J Clin Pathol. 1982 Oct;35(10):1134-7. doi: 10.1136/jcp.35.10.1134.
Abstract
A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ELISA method. Eleven sera that reacted with both antigens in AGDD were all positive against antigen 1 in both forms of ELISA, but two failed to react with antigen 2 in the standard ELISA and three failed to react with this antigen in the rapid method. Thirteen AGDD-negative sera were also negative in both forms of ELISA. The rapid ELISA provides a sensitive and reproducible test for routine serological investigation of allergic aspergillosis.
摘要
将一种组分温育期缩短至1小时的快速酶联免疫吸附测定（ELISA），与琼脂凝胶双向扩散法（AGDD）以及标准ELISA程序进行比较，以检测28例疑似变应性曲霉菌病的哮喘患者体内抗烟曲霉菌的抗体。使用两种烟曲霉菌抗原，快速ELISA与AGDD及标准ELISA方法的检测结果相当。在AGDD中与两种抗原均发生反应的11份血清，在两种形式的ELISA中针对抗原1均呈阳性，但有2份血清在标准ELISA中未与抗原2发生反应，3份血清在快速法中未与该抗原发生反应。13份AGDD阴性的血清在两种形式的ELISA中也均为阴性。快速ELISA为变应性曲霉菌病的常规血清学检测提供了一种灵敏且可重复的检测方法。
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