Hirai A, Terano T, Hamazaki T, Sajiki J, Saito H, Tahara K, Tamura Y, Kumagai A
Thromb Res. 1983 Jul 1;31(1):29-40. doi: 10.1016/0049-3848(83)90005-1.
The effects of Moutan Cortex and one of its major components, paeonol, on platelet aggregation and arachidonic acid (AA) metabolism in human platelets were studied. One week oral administration of water extract of Moutan Cortex [Moutan Cortex (w), 3 g/day] significantly reduced platelet aggregation and thromboxane B2 (TXB2) formation induced by collagen, epinephrine and ADP. Paeonol dose-dependently inhibited ADP and collagen induced platelet aggregation in vitro. Moutan Cortex (w) and paeonol dose-dependently inhibited the conversion of exogenous [14C]AA to [14C]heptadecatetraenoic acid [( 14C]HHT) and [14C]TXB2 by washed human platelets, while both of them increased its conversion to [14C]12-hydroxy eicosatetraenoic acid [( 14C]12-HETE). High dose of Moutan Cortex (w) inhibited the release of [14C]AA from prelabeled platelets in vitro, while paeonol did not. These results suggest that a reduction in platelet aggregation by the oral administration of Moutan Cortex might be ascribed to a decrease in thromboxane synthesis and that paeonol might play an important role in the antiaggregatory effect of Moutan Cortex because of its potent inhibitory effect on platelet aggregation and thromboxane formation.
研究了牡丹皮及其主要成分之一丹皮酚对人血小板聚集和花生四烯酸（AA）代谢的影响。连续一周口服牡丹皮水提取物[牡丹皮（水），3克/天]可显著降低胶原、肾上腺素和ADP诱导的血小板聚集以及血栓素B2（TXB2）的形成。丹皮酚在体外剂量依赖性地抑制ADP和胶原诱导的血小板聚集。牡丹皮（水）和丹皮酚剂量依赖性地抑制洗涤后的人血小板将外源性[14C]AA转化为[14C]十七碳四烯酸[（14C）HHT]和[14C]TXB2，而二者均增加其向[14C]12-羟基二十碳四烯酸[（14C）12-HETE]的转化。高剂量的牡丹皮（水）在体外抑制预标记血小板释放[14C]AA，而丹皮酚则无此作用。这些结果表明，口服牡丹皮导致血小板聚集减少可能归因于血栓素合成的减少，并且丹皮酚可能因其对血小板聚集和血栓素形成的强效抑制作用而在牡丹皮的抗聚集作用中发挥重要作用。
